| Objective:To explore the effect of antiviral agent lamivudine in enhancing theradiosensitivity of human hepatitis B virus-related hepatocellular carcinoma cell lineHepG2.2.15cell and preliminarily investigate the possible radiosensitizingmechanisms．Methods:Fluorescent quantitative PCR assay for detection of virus inhibitory effect oflamivudine(0,2.5,5,10,25μg/mL)in hepatitis B virus-related hepatocellular carcinomaHepG2.2.15cells;The influence of lamivudine on the radiosensitivity of HepG2.2.15cells were detected by colony formation assay; The apoptosis rate and change incell cycle of HepG2.2.15cells were examined by flow cytometry;RT-PCR,Cellimmunohistochemical and Western blot were used to detect the expression level ofCyclinD1in HepG2.2.15cells.Results:Fluorescence quantitative PCR detection results showed that lamivudine caninhibit the replication of the hepatitis B virus in HepG2.2.15cells,and the bestinhibition effect in10μg/mL at48h; colony formation assay showed that lamivudinesignificantly reduced the clonogenic rate of HepG2.2.15cells after irradiation with aSER of1.224; Flow cytometry analysis showed that the proportion of G0/G1phaseand the apoptosis rate increased in the group of lamivudine treat with irradiation (p＜0.05);Combining lamivudine with irradiation decreased the expression of CyclinD1mRNA and CyclinD1protein in HepG2.2.15cells.Conclusion:Antiviral agent lamivudine could both inhibit HBV replication and enhance theradiosensitivity of HepG2.2.15cells in vitro.Its mechanism may be associated withdown-regulation the expression of CyclinD1gene that lead to block of G0/G1phaseand enhancing radiation induced apoptosis of HepG2.2.15cells．... |
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