BubR1Gene Expression In Liver Cancer Cells And Its SiRNA Transfectant’s Building

Objective:Research BubR1expression differences in HBV positive and negative HCCcells. Design and select siRNA sequences that could inhibit the expression of BubR1.Then transfect the siRNA into HepG2.2.15cells by Lipofectamine TM2000andresearch the effect of hepatoma carcinoma cells.Methods:Applying the technology of PCR and Western blot test BubR1expression inHBV positive（HepG2、SMMC-7721、Huh7） and negative iver cancer cells（HepG2.2.15、Hep3B）. Choose the most representative cells for subsequentexperimental study. Designed pEGFP-SiBubR1plasmid transfected into HepG2.2.15cells.To detect the influence of HepG2.2.15cells after transfection.Results:RT-PCR technology detection results shown that HepG2.2.15cells the HBVpositive liver cancer cell express the highest BubR1level；Western-blot detectionresults shown the same result；Transfection efficiency was demonstrated high byobserving the greenfluorescence through fluorescence microscope. The expression ofBubR1was degraded obviously by the detection of RT-PCR and Western-blot aftertransfection. Especially degraded after siRNA-BubR-3transfection.Conclusion:BubR1over expression in HBV positive liver cancer cells. RT-PCR andWestern-blot detection show that HepG2.2.15cells express the highest BubR1level.SiRNA can significantly reduce HepG2.2.15cells after transfection. SiRNA-BubR1-3transfection group of inhibitory effect is most obvious. BubR1is obvious correlation with HBVpositive liver cancer cells. The results help to further study of HBX gene and BubR1correlation and its effects on HCC.