Explore On The Role Of Adjusting Gastric Motion By Acupuncture At "Zusanli" Point And The SP、MTL Of NRM

Objective Establish gastric function and inhibition models induced, using acupuncture at the “Zusanli” point, observation gastric peristalsis wave the change of SP and MTL in gastric antrum and NRM, explore the role of NRM and adjusting gastric motion by acupuncture at " Zusanli " point.Methods50SD rats, completely randomly divided into5groups. Respectively for A:Blank control group, B:Gastric function hyperthyroidism group, C:Gastric function inhibition group D:Gastric function hyperthyroidism+Acupuncture group E: Gastric function inhibition group+Acupuncture group. By using of physiological signal analysis system way record MP150CE record the stomach movement wave of every group, udeing enzyme league immune method (ELISA) test the content of MTL and SP in the gastric antrum; useing immunohistochemistry methods were used NRM testing in expression of MTL and SP. Results Blank control group rats tail intravenous saline, stomach movement volatility and frequencies are no significant changes. Rats tail intravenous metoclopramide stomach movement after increased speed up the frequency, wave amplitude, gastric function in hyperthyroidism state, acupuncture at "Zusanli" can make its stomach movement bobo big decline, frequency to slow, tail intravenous atropine or metoclopramide all can make gastric antrum SP different content of the organization in a reduced, acupuncture can make gastric peristalsis rats induced gastric antrum down the content in the organization, and make the SP gastric peristalsis inhibiting the rat gastric antrum tissue of the increased levels SP.And rats tail intravenous atropine stomach movement after big decline slowing, frequency wave.gastric function in the suppression condition. acupuncture at “Zusanli" Point can make its stomach movement, speed up the frequency of bobo increased; And each rat gastric antrum organization MTL content is no significant differences. The tail intravenous metoclopramide or stomach movement caused by atropine hyeorthyroidism or inhibit, the expression of MTL and SP in NRM is reduced, acupuncture at "Zusanli" Point can make gastric peristalsis rats’ MTL expression is induced and SP expression is reduced in NRM, make Gastric peristalsis inhibit rat’s the expression of MTL and SP in NRM are increased. Conclusion (1) Acupuncture at "Zusanli" Point has obvious two-way adjustment function.(2) The adjustment of the movement of the stomach acupuncture can through affecting NRM’the expression of MTL and SP in the brain stem. The possible mechanism Of adjusting gastric motion by acupuncture at "Zusanli" Point is: acupuncture signal through the nerve conduction induce the expression of MTL and SP in NRM,to change the content of SP, MTL in gastric antrum adjust gastric movemen.