Application Of Laser Tweezers Raman Spectroscopy In Vivo Rat

The advantage of Raman spectroscopy is a non-invasive, low-destructive, do not have the reagents and a high degree of automation.it was greatly applied in medical diagnosis. Laser tweezers Raman spectroscopy is a new biological photonic technology that is combination of optical trapping and Raman spectroscopy. This technique has been used to study single living suspension cells. With this system, high-quality and sensity Raman spectra were obtained from single optically trapped biological cells in suspension. Raman spectroscopy is a promising tool for studying living process of the single cell and medicine diagnosis. Currently, Raman spectroscopy was applied in analysis of single cell.Diabetes is a high prevalence of complications of chronic disease, but there are50%to70%of patients do not fell obvious symptoms. It was occurd unconsciously and developed gradually.so diabetes was called "invisible killer". Because diabetes is often accompanied by a variety of complications such as tissue necrosis, myocardial, it waas the third largest non hereditary diseases from cancer, cardiovascular and cerebrovascular diseases. Diabetes threatens seriously the health and life of human being, and therefore effectively prevent complications reduce the mortality rate of diabetes is very important. At present, detection of blood glucose is one of the important goals, the method of testing blood glucose is that blood from the body were analyzed by biochemical tests.In vitro experiments is not true in vivo under physiological conditions;frequent blood sampling is spending too much time and increasing the patient’s suffering and economic burden. However, because the low glucose content and small range in the tissue fluid and blood, a lot of water in the organization, the glucose absorptivity is far less than the water absorptivity and changes in blood glucose concentration cause effective weak signal. The accuracy and reliability of Raman spectroscopy of the measurement blood glucose non-invasive, there is a certain difficulty. Raman spectra of normal WBC and DM WBC in vivo were studied by laser tweezers combined with Multivariate statistic analysis method.Raman spectrum and Multivariate statistic model was found by the principal components analysis (PCA).the result of Raman spectrum suggested:1.DM WBC and normal WBC could be differentiated with PCA. Diagnostic specificity and sensitivity are come to98%.2.The protein vibration bands at1302cm-1in DM WBC in vivo rat showed that the concent of protein in DM WBC are higher than normal WBC.3.The Raman intensities at O-P-O of DNA and Amide Bands of DM cell are significantly higher than normal cell,which showed that the changes in comformation of O-P-O、the backbone and hydrogen bonds of protein and two comformation of protein.The lack of oxygen supply is the most important pathophysiological basis of tissue necrosis and other complications. blood cells is the main carrier of oxygen supply, abnormal structure and function of blood cell is the most direct cause of the lack of oxygen supply. Since the mature red blood cells change their shape could through the microvascular.therefore, a good red blood cell deformability is the most important guarantee to maintain the the microcirculation material with oxygen. Studies have shown that the decreasing of blood cell deformability cause microcirculation disorders and local tissue ischemia and hypoxia in diabetes and a variety of diseases, it is the main reason for diabetes complications can happen easily. In recent years, the blood cell deformability as the pathogenesis of diabetic microvascular disease from the perspective of hemorheology, an objective index of efficacy of principle and preventive measures important parameters has been increasing attentied. But the research process of blood cells in vitro in the past often is trouble, which is bound to changing their living environment that can not objectively reflect the actual situation.it is especially necessary in situ non-destructive research.Using non-destructive method to study the in situ blood cell composition of the diabetic mice and its composition changes in different states, this method has not been reported as far as we know. Raman spectra of normal RBC and DM RBC in vivo were studied by laser tweezers combined with Multivariate statistic analysis method.Raman spectrum and Multivariate statistic model was found by the principal components analysis (PCA).the result of Raman spectrum suggested.1.1.DM RBC and normal RBC could be differentiated with PCA. Diagnostic specificity and sensitivity are come to98%.2. The Raman intensities at ferrohemoglobin and phenylalanine of DM cell are significantly higher than normal cell,which showed that glycosylation ferrohemoglobin, metabolism abnormal.3.7he ratios of showirg the Oxgen-carring capacity I1635/I1550in DM were less than those in normal. It is that the Oxgen-carring capacity of RBC in DM was lower than that in normal subjects;7he ratios of showirg the fluldity I1126/I1080in normal subjects were less than those in DM. It is that the fluidity of erythroeyte membrane in DM was lower than that in normal subjects. The above conclusions showed that Raman optical tweezers technology in a quick and effective tool that studies of cell physiological and biochemical changes. It is expected to become the advanced tools that detection and diagnosis of all kinds of living cells at the molecular level.