The Effect Of Blood Storage Time On Blood Components And The Amount Of Residual White Blood Cells In Less White Blood Cell Suspension Red Blood Cell

According to the statistics of Henan provincial blood information management system,the annual demand for clinical blood is increasing at a rate of 10% per year.A total of 106 million 620 thousand people in the Henan province and there were1001512 blood donors in 2015,the blood donation rate was 9.39‰.In order to meet the growing demand for clinical blood,we must actively carry out the composition of blood transfusion.The preservation time of whole blood has become the key point in the preparation of blood components,and it has become one of the key factors to carry out this work.The rational use of blood resources and reduce the adverse reactions caused by blood transfusion have become a hot topic in the field of blood transfusion and clinical care.The main cause of serious adverse reactions to blood transfusion is the white blood cells in the blood.At home and abroad,it has been proved that infusion of the blood without white blood cells can avoid or reduce the occurrence of adverse transfusion reactions.At present,the blood with filtering out white blood cells has been widely used in the clinical application in many countries,including Finland,Japan.The application of this technique has greatly reduced the incidence of non-hemolytic transfusion adverse reactions.The method has been gradually popularized in our country and accepted by the vast majority of blood transfusion institutions and hospital clinical medical departments.With the application of less white blood cell suspension red blood cell is more and more extensive and its blood products are more and more.Based on the above research background,we carried out this study.ObjectiveThis study intends to explore the effect of blood storage time on blood components and the amount of residual white blood cells in less white blood cell suspension red blood cell and to evaluate the best preservation time,so as to improve the utilization rate of blood,blood,blood products save improve purity,reduce sideeffects and improve the curative effect.MethodsWe randomly selected 1500 voluntary blood donors from January 2015 to December in line with the "blood donors health inspection requirements".There were960 males and 540 females.Using Baxter,Fresenius and MacoPharma,the production of a one-time filter with embedded bottom and top,to collect whole blood,respectively.Blood samples were collected at 22±2 ? for 6~22 hours,and the number of white blood cells and platelets in whole blood was measured every 4 hours.The whole blood was preserved for 6~22 hours,and the white blood cells were removed from the whole blood every 4 hours.The number of white blood cells was counted by Onyx cell counting instrument,Beckman counter and BD biological science counter,respectively.The number of white blood cells and platelets were expressed as mean ±standard deviation.The difference between the amount of white blood cells and platelets at different time points was compared by repeated measures analysis of variance.Six hours whole blood preservation time was taken as reference,and the number of white blood cells(or platelets)measured at different time points and the number of white blood cells(or platelets)measured by 6h were compared by paired t test.Single factor analysis of variance was used to analyses the number of white blood cells at each time point by three different white cell count methods(Onyx cell count,Beckman count,BD biological science counter).Results1.Baxter blood collection system: The number of white blood cells was the highest in 14 hours whole blood preservation time,and the results of repeated measures analysis showed that the number of white blood cells in the whole blood was different under different preservation time,and the difference was statistically significant(P<0.05);the number of platelet was the highest in 14 hours whole blood preservation time,repeated measures analysis of variance showed the number ofplatelets in whole blood under different preservation time is different,and the difference was statistically significant(F=9.945,P<0.001);single factor analysis of variance showed that there were significant differences in the number of white blood cells,which were detected by Onyx cell counting instrument,Beckman counter and BD biological counting instrument(P<0.05).2.Fresenius blood collection system: The number of white blood cells was the highest in 14 hours whole blood preservation time,and the results of repeated measures analysis showed that the number of white blood cells in the whole blood was different under different preservation time,and the difference was statistically significant(P<0.05);the number of platelet was the highest in 10 hours whole blood preservation time,repeated measures analysis of variance showed the number of platelets in whole blood under different preservation time is different,and the difference was statistically significant(F=1.924,P=0.122);single factor analysis of variance showed that there were significant differences in the number of white blood cells only in the whole blood preservation time of 10 h,which were detected by Onyx cell counting instrument,Beckman counter and BD biological counting instrument(P<0.05).3.MacoPharma blood collection system: The number of white blood cells was the highest in 14 hours whole blood preservation time,and the results of repeated measures analysis showed that the number of white blood cells in the whole blood was different under different preservation time,and the difference was statistically significant(P<0.05);the number of platelet was the highest in 10 hours whole blood preservation time,repeated measures analysis of variance showed the number of platelets in whole blood under different preservation time is different,and the difference was statistically significant(F=15.529,P=0.122);single factor analysis of variance showed that There was significant difference in the number of white blood cells only when the whole blood preservation time was greater than 14 h,which were detected by Onyx cell counting instrument,Beckman counter and BD biological counting instrument(P<0.05).4.The number of platelets is basically the same in the blood collected by Baxter,Fresenius and MacoPharma systems,single factor analysis of variance showed nosignificant difference(F=0.123,P=0.884);However,the number of white blood cells was not consistent,and the difference was statistically significant(F=4.392,P=0.013).5.Repeated measures analysis of variance showed that the number of residual white blood cells in less white blood cell suspension red blood cell was different in different time of whole blood preservation(P<0.05).Conclussion1.the preservation time of whole blood can affect the number of white blood cells and platelets,prolonged storage time can lead to an increase in the number of platelets in whole blood.2.The preservation time of whole blood could affect the number of residual white blood cells in less white blood cell suspension red blood cell.3.Different blood collection systems had no effect on platelet count in whole blood,but there were differences in the number of white blood cells.4.The number of white blood cells detected by Onyx cell counting instrument,Beckman counter and BD biological science counter was different.5.Taking into account the quality of less white blood cell suspension red blood cell after the filtration of white blood cells,the preservation time of 12-20 hours may be the best time for whole blood preservation.