Preliminary Study On The Mechanism Of Decline Of White Blood Cell In Early Stage Of Urosepsis

Objective:To simulate uroseptic shock following upper urinary tract endoscopic lithotripsy (UUTEL), we built animal model via creating high pressure in renal pelvis. Our objective is to explore the mechanism of early white blood cell(WBC) decrease and provide theoretical basis for WBC decrease as a predictor for urosepsis.Methods:15 New Zealand rabbits were assigned in to three groups. Group A was the control group and their bone marrow smear, complete blood test and liver, kidney, spleen and heart were examined with no intervention. In experimental group B and C, uroseptic model were built. Group B's bone marrow smear, complete blood test and liver, kidney, spleen and heart were examined 1 hour after the operation and group C was examined the same way but 2 hours after the operation. We studied the difference of bone marrow among three groups and observed whether there was any inflammation cells infiltration in peripheral tissue 1 hour and 2 hours post-operatively. And we also intended to find out the whereabouts of WBC 1 hour and 2 hours post-operatively and the variation of WBC and neutrophils.Results:In complete blood test, compared to control group A, WBC and neutrophils were dropped significantly (p<0.05) and reached their lowest 2 hours post-operatively in group B and C. In bone marrow smear, compared to control group A, there was no significantly difference 1 hour and 2 hours post-operatively in group B and C. In pathology examination, inflammation cell infiltration were much severe in group C than in group B, and less severe in group A.Conclusions:WBC decrease 2 hours after uroseptic model creation is not caused by bone marrow suppression and could be related to WBC infiltration in tissue due to marked inflammation in peripheral organs.