The Influence Of Gujiankeli To VEGF、NOS And ALP In Promoting Early Fracture Healing

ObjuctiverTo study the Gujiankeli in the promotion of bone health of early fracture healing of vascular endothelial growth factor (VEGF) nitric oxide synt-hase (NOS) alkaline phosphatase (ALP) and its isomer activity, to explore the correl-ation between VEGF NOS and ALP, to further clarify the Gujiankeli to promote bon-e health of early fracture healing mechanism.Methods:Build50rabbits were randomly divided into Gujiankeli group (hight medium low), Shangkejiegu group and blank group, respectively administration after the first day of taking peripheral1,4,7,10,14,21,28day blood,using enzymelinked immunosorbent assay and chemical colorimetric determination of VEGF content total NOS and inducible nitric oxide synthase (iNOS) and nitric oxide synthase native (cNOS) and ALP activity.Result:(1) Healing of early serum VEGF levels (in OD value indicated) in the1,4,14,21day, Gujiankeli medium dose group and the blank group there was a significant difference (p<0.01), in the7d significant difference (p<0.05); and Shangkejiegupian group, in the14d there was a significant difference (p<0.01); in7d and28d there is asignifica nt difference (p<0.05);(2) Serum NOS activity changes in the4d,7d,14d, when Gujiankeli medium dosegroup group and Shangkejiegupia group and blank group there was a significant difference (p<0.01); in the21d, when the Gujiankeli medium dose group and the blank group there was a significant difference (p<0.01), while in1day there are significant differences (p<0.05);(3) Serum iNOS activity changes in7day, when Gujiankeli medium dose and Shangk-ejiegu group have a very significant difference (p<0.01), in the21day, w hen there are significant differences (p<0.05); and the blank group in7,21day is a very significant difference (p<0.01), in the1d,4d, when there are significant differences (p<0.05);(4) Changes in cNOS activity in serum, in4,7,14day, and when the Gujiankeli medium dose and Shangkejiegu group and blank group subjects compared with bone pieces have a very significant difference (p<0.01); in the21day, when Gujiankeli medium dose group and the blank group compared to the very significant differences (p<0.01), in1day, when there are significant differences (p <0.05).(5) Serum ALP activity of change, Gujiankeli dose group Shangkejiegupian Blank group in7,14,21,28day very significant compared difference (p<0.01); Gujiankeli dose group and blank group group in4day when compared with significant difference (p<0.01).Gujiankeli low group and high dose group compared with other group no obvious difference.Conclusion:Early fracture healing in serum VEGF、NOS and ALP in the different changes in different periods;Gujiankeli can improve VEGF expression activation NOS and ALP activity, This may be the generation mechanism of clinical efficacy.