Study On The Serum IL-6、IL-8、TNF-α Levels In Patients With Chronic Obstructive Pulmonary Disease With Type2Diabetes

Background: Chronic obstructive pulmonary disease(COPD) ischaracterized by progressive, not completely reversible restrictedairflow，is chronic inflammation of respiratory system disease.Theinfection is the main cause of the AECOPD period.Diabetes is a systemicmetabolic syndrome, characterized by chronic blood glucose levelsrise,caused by the genetic and environmental factors.Both are the mostcommon clinical disease.Because of the quantity of case，high fatalityratio，heavy social economic burden, have become an important publichealth problem.COPD wiht type2diabetes will increase the difficulty ofthe treatment. At the same time also significantly reduce the patient’squality of life. So the study of the mechanism has the vital significance toclinical practice。Objective：Study on the serum IL-6、IL-8、TNF-α expression levelsin patients with chronic obstructive pulmonary disease with type2diabetes, to further discuss the pathogenesis.Methods:Patients with acute phase COPD、 normal controls、patients with type2diabetes、patients with acute phase COPD with type2diabetes were enrolled in the study,every group is constituted of20person.The participants lung functions were measured byspirometry.Peripheral blood samples were taken at both attack and stablestage of the disease. The concentrations of serum IL-6、IL-8、TNF-α weremeasured according to manufacture’s instruction by ELISA ki. Results：（1）The IL-6、IL-8、TNF-α in blood are higher in the acutephase COPD patinets and the stable phase COPD patinets than thehealthy control group.It has significant difference (P<0.01). IL-6、IL-8、TNF-α are involved in the COPD airway inflammation.（2）The IL-6、IL-8、TNF-α in blood are obviousely higher in the patients with acutephase COPD with type2diabetes than with acute phase COPD andpatients with type2diabetes.It has significant difference (P<0.01)（.3） Itis a positive correlation between IL-6level and HbAlc (r=0.36, P <0.01), IL-8level and HbAlc have no correlation.It is a some degree ofcorrelation between IL-8level and HbAlc(r=0.35，P<0.01).（4）In thediabetes group, FEV1%,a lung ventilation function index,is negativelycorrelated to HbAlc（r=-0.320、P<0.01）.Obstructive ventilation functiondecline significantly.DLCO/VC，the lung function indexes of dispersion,is negatively correlated to HbAlc（r=-0.783，P<0.01）.Conclusions:(1)The serum IL-6、IL-8、TNF-α are higher in the acutephase COPD patients and the stable phase COPD patients than thehealthy control group.It has significant difference.COPD is chronicairway inflammation，commonly participated of a variety of cell factors.Those cell factors, may is one of important factors, that a cause of lunginflammatory cells invasionand the lung tissue structure destroy.(2) Theserum IL-6、IL-8、TNF-α level are obviousely higher in the patients withacute phase COPD with type2diabetes than with acute phase COPD andpatients with type2diabetes.The patients with acute phase COPD withtype2diabetes pathogenesis may be the immune response commonlyparticipated of IL-6、IL-8、TNF-α.(3) It is a positive correlation betweenIL-6level and HbAlc, IL-8level and HbAlc have no correlation.It is asome degree of correlation between IL-8level and HbAlc.When acute phase COPD with type2diabetes, high blood glucose level influence theproduction of IL-6, TNF-α.(4) In the diabetes group, FEV1%expected value,a lung ventilation function index,is negatively correlatedto HbAlc.Obstructive ventilation function decline significantly.DLCO/VC， the lung function indexes of dispersion, is negativelycorrelated to HbAlc.Show that for high blood glucose can lead tofurtherly worsen lung function in patients with COPD.