| Objective:To find differential expression protein in patients with T cell Non-Hodgkin lymphoma(T-NHL) by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry(SELDI-TOF-MS) technique, while study their clinical application value and prospect.Methods:Serum protein of36T cell lymphoma patients were detected by the SELDI-TOF-MS technique and weak cation exchange(wcx2) chip. Lactate dehydrogenase (LDH) was detected by biochemistry method. Beta-2-microglobulin(β2-MG) was detected by enzyme-linked immunesorbent assay(ELISA).The significant different protein spectrometry were analyzed between DLBCL patients and T-NHL patients. The correlation analysis with protein spectrometry, disease staging,LDH and β2-MG were analyzed with spearman.Results:9potential candidate proteins, including the peak intensity of1142.67、1451.43、1472.49、1512.03、3194.22、3267.41、3933.86、4593.12and9182.24.were identified in T-cell lymphoma patients. The9protein marker had no contact with disease staging of T-NHL(p>0.05). The protein marker of4593.12and9182.24were high level in T-NHL patients. LDH in these two protein markers positive group(2.9082E2±2.99497E1、2.8394E2±1.0094E2) was higher than that in negative group(1.6922E2±5.5424E1、1.6950E2±5.9245E1)(t1=-3.199, P1=0.004; t2=-2.378, P2=0.026),and LDH was positive correlation with these two protein spectrometry (r1=0.265, r2=0.178, P<0.01). There was no statistically significant difference of β2-MG between these two protein markers positive group and negative group(p>0.05). The other7protein markers were low level in T-NHL patients, and there was no statistically significant difference of LDH and β2-MG in these8protein markers (p>0.05).Conclusion The protein marker of4593.12and9182.24may be the specific Serological markers to identify T-NHL. The combination of these two protein marker and LDH may assess the tumor load, and provide guiding value for clinical treatment. |
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