The Expression And Significance Of TNF-a And VEGF In Budd-chiari Syndrome Rat Model Of Liver

Objective:1. Build a reliable、stable rat model of Budd-Chiari syndrome, study its development and changes;2.To study the expression and significance of TNF-a、 VEGF in different time periods in Budd-Chiari syndrome rat model of liver.Methods:1. Use the inferior vena cava constriction production Budd-Chiari syndrome rat model:Separation of rat inferior vena cava, with the0silk thread ligation the inferior vena cava and L shaped23G blunt Scalp, take out the blunt Scalp, Inferior vena cava cross-sectional area narrowing of approximately80%.2. Select120healthy adult female SD rats (provided by Experimental Animal Center of Henan Province) for the experimental study, the weight is about400g, Were randomly divided into Group A:normal group, group B:sham group;Group C: operation group, model group. N=40, control group not given surgical treatment; The sham operation group only received surgical separation of the liver after the inferior vena cava; Operated group liver after coarctation of inferior vena cava to copy the model of Budd-Chiari syndrome. after the Budd-Chiari syndrome model established, in the different time points1w,4w,8w,12w Each group were sacrificed8, To extract the inferior vena cava blood antiserum, Determination of serum levels of TNF-a and the levels of VEGF by enzyme-linked immunosorbent assay (ELISA), For morphological examination of liver tissue, And detected using immunohistochemical techniques in rat liver TNF-a, VEGF expression levels.Statistical analysis using the statistical software SPSS10.0, x±s represents the experimental data.Results:1. Use the inferior vena cava constriction production Budd-Chiari syndrome rat model, the mortality rate is15%.The first week of the rats began to produce ascites; The fourth week of color Doppler ultrasound shows:the abdominal cavity has begun to significantly fluid areas, liver, and spleen enlargement, inferior vena cava and main hepatic vein thrombosis, portal vein dilatation within the liver parenchyma can be seen widely traffic open, the imaging findings conform BCS.2.The group A and group B serum levels of TNF-a at all time points are relatively stable, no significant change with time, the trend of group A and group B is the basically same, Group B does not due to the surgical interference at each time point than in group A were have significantly different (P>0.05)..group C serum TNF-a showed an increasing trend at all time points, The overall level of can be seen than in group A (P<0.01) and group B(P<0.01) in each point time, Combination of pathological changes in the rat liver, the expression of TNF-a in serum and liver fibrosis are related.3.group A and B serum levels of VEGF at different time points are relatively stable, do not significantly with the time change, the trend of group A and group B are the same basically, Comparison at each time point VEGF levels in group B at all time points than in group A,were not significantly different (P>0.05). group C serum VEGF at all time points showed an increasing trend, the overall level of each point in time can be seen that are greater than in group A (P<0.01) and group B (P<0.01), combined with the pathological changes of the rats liver, positively related to the expression of VEGF in serum and liver fibrosis.4.TNF-a expression within the liver tissue in group C in the first week began to increase, with gradually increased trend, Each time point of TNF-a expression levels are higher than those in group A and group B, statistical significant difference (P<0.01). Group A and group B liver tissue TNF-a expression level is basically unchanged, at each time point of TNF-a expression level was no significant difference (P>0.05).5. VEGF expression within the liver tissue in group C in the first week began to increase, with gradually increased trend, Each time point of VEGF expression levels are higher than those in group A and group B, statistical significant difference (P <0.01), VEGF levels in the liver tissue of group A and group B did not change, compare the VEGF levels at each time point no significant difference (P>0.05).6. liver histopathological examination:Group A and group B of rat liver tissue were normal under the microscope at each time point. Group C rats at8w,12w can seen the liver tissue lobular architecture disorders under microscope, A wide range of fatty degeneration of liver cells, partial necrosis, portal area to expand, fibrosis, The coarse fibrous septa were formed and extension of split liver lobule, Hepatic fibrosis with time increased.Creativity:1. First established a rat model of Budd-Chiari syndrome with ligation of the Inferior vena cava;2. First detection the expression of TNF-a and VEGF in liver of rat model of Budd-Chiari syndrome;3. First observe the dynamic process of Budd-Chiari syndrome of hepatic fibrosis in rats.Conclusions:1. Use the inferior vena cava constriction production Budd-Chiari syndrome rat model, Such a method of modeling a high success rate, the mortality is low, the cycle is short, simple, low cost, suitable for large-scale study required;2. Budd-Chiari syndrome rat model of serum and liver TNF-a and VEGF levels andtime of onset of Budd-Chiari syndrome was a significant positive correlation; 3. Budd-Chiari syndrome rat model of liver fibrosis and liver TNF-a and VEGF levels were positively correlated, TNF-a and VEGF were expression in the liver tissue is meaningful for the Budd-Chiari syndrome of the severity of the condition.