| Objective:To identify the main chemical composition, glycyrrhizic acid, ammonium glycoside and assay the content of total flavonoids of Abrus precatorius L. Methods:(1) Chemical reaction was used to identify the chemical composition of the aqueous extract and the ethanol extract of Abrus precatorius L.(2) TLC and HPLC were used for the qualitative and quantitative analysis of glycyrrhizic acid.(3) Spectrophotography was used to assay the content of total flavonoids of Abrus precatorius L. Results:(1) Abrus precatorius L. contains glycoside or polysaccharide, organic acid, phenols, amino acid, flavonoids, protein, steroid saponin or triterpene saponin.(2) Glycyrrhizic acid was not detected in Abrus precatorius L.(3) Abrus precatorius L.contents total flavonoids is0.27%. Conclusions:Abrus precatorius L. contains many kinds of chemical composition. Objective:To investigate the effects and mechanisms of aqueous extract of Abrus precatorius L. on relieving alcoholism and alcohol, CC14, acetaminophen, D-galactosamine-induced acute hepatic injury in mice. Methods:(1) mice were devided into five groups and were administrated with high, medium, low-dosage of APL, haiwangjinzun tablets and NS respectively for six days. After30minutes of the last administration, the mice were given56%alcohol every20minutes until they were died. Record the drunk (frighting reflex disappear) incubation period, drunk dosage, time of death and the lethal dose of alcohol.(2) The models of alcohol, CCl4, AP and D-GalN-induced acute hepatic injury in mice were used to study the effect of APL. Models were established and drug was deal with before the activities of ALT, AST in serum and the amounts of SOD, MDA, GSH, GSH-PX, ADH and ALDH in hepatic tissue were measured. Results:(1) The incubation period of drunkenness was extended significantly when administrated APLH before drunkenness (P<0.05).(2) In various types of acute hepatic injury’, APLH could significantly decrease the elevation of AST and ALT in serum in acute hepatic injury (P<0.01). In addition, APLH could obviously promote the content of SOD, GSH, GSH-PX, ADH and ALDH in hepatic tissue, and decrease the MDA content significantly (P<0.05或P<0.01)Conclusions:APL could prevent drunkenness and had effect on protecting alcohol, CCl4, AP, D-GalN-induced acute hepatic injury in mice. The mechanisms may be related to attenuating free radical and inhibiting the lipid peroxidation. Objective:To investigate the effects and mechanisms of aqueous extract of Abrus precatorius L. on alcohol-induced chronic hepatic injury in rat. Methods: the model of alcohol chronic hepatic injury in rats was established by alcohol, and treated with APL at the same time. Alcohol and drugs was gived everyday respectively, after24weeks all rats were deal with as follow:(1) The serum and hepatic tissue were taken when rats were sacrificed, and the activities of ALT, AST, SOD, MDA, GSH, GSH-PX, ADH and ALDH in serum and hepatic tissue were measured.(2) The hepatic tissue of every rat was taken and soaked in10%neutral formalin solution. After24hours hepatic tissues were paraffin-embedded and formalin-fixed, hematoxylineosin stain and immunohistochemistry were used to examine the degree of injury in rats.(3) The expressions of β-Catenin, CyclinD, Col-Ⅰ mRNA in hepatic tissue were determined by Q-PCR and the expressions of β-Catenin protein were determined by immunohistochemistry. Results:(1) The activities of ALT, AST and the content of MDA in serum and in hepatic tissue were decreased significantly in APLH groups.(2) APLH could relieve necrosis and degeneration of hepatic cell, and recover its fundamental structure.(3) The expressions of β-Catenin,CyclinD1, Col-Ⅰ mRNA and β-Catenin protein were down-regulated by APLH.Conclusions:APL could protect alcohol-induced chronic hepatic injury in rat. The mechanisms may be related to improving the ability of eliminate free radical in liver and inhibiting the expression of β-Catenin, CyclinD1, Col-Ⅰ in hepatic cell. |
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