The Association Between Single Nucleotide Polymorphisms Of Macrophage Migration Inhibitory Factors Rs755622and Preeclampsia

Objective To investigate the association between single nucleotide polymorphisms of macrophage migration inhibitory factors rs755622and the pathogenesis of preeclampsia. Methods165preeclampsia pregnant women were recruited,who can be divided into mild preeclampsia group(72cases) and severe preeclampsia group(93cases).120normal pregnant women were selected for the control group in the same period. Single nucleotide polymorphisms (SNP) of MIF-rs755622were typed by polymerase chain reaction-restriction fragment length polymorphism. Then the differences of genotype frequencies and the allele frequencies in each group were compared. The levels of fasting blood glucose (FBG), insulin (FIN) and homeostasis model assessment of insulin resistance (HOMA-IR) were determined in each group. Then the FPG, FIN and HOMA-IR levels of different genotype frequencies were compared in every group. Results Three genotypes were detected in MIF-rs755622site(GG,GC and CC).There were no statistical differences in genotype frequencies and allele frequencies between the preeclampsia group and the control group (P>0.05); there were also no statistical differences in genotype frequencies and allele frequencies between the mild preeclampsia group and the serve preeclampsia group (P>0.05). The severe preeclampsia patients and mild preeclampsia patients with CG and CC genotypes had higher fasting insulin levels and higher HOMA-IR compared with the GG genotype (t=3.17-9.74, P<0.05). Conclusion The present study suggests that the MIF-rs755622G/C SNP is associated with insulin resistance in preeclampsia patients. The GC and CC genotypes increase the degree of insulin resistance, but The MIF-rs755622G/C SNP was not associated with the genetic susceptibility of preeclampsia. The MIF-rs755622G/C polymorphism was not the susceptible site of preeclampsia in Han people of north China.