The Influence Of Sinomenine On Invasion And Metastasis Of Hepatocellular Carcinoma Cells HepG2

Background:Sinomenine is a kind of biological activity alkali which has been picked up from QingFengTeng (Sinomenium acutum Rehder&E.H. Wilson), and widely used for the treatment of rheumatoid arthritis. In recent years, it was found sinomenine play an important role in inhibit tumor cell growth. This experiment is to expplore the possible mechanism of sinomenine’s ability to inhibit tumor cells invasion and metastasis by invastigation on HepG2, a human liver cancer cells.Aim:To study the inhibition of sinomenine to HepG2by the different concentration, and to detect the effect of sinomenine on HepG2cell adhesion, invasion and metastasis in vitro. Another objective is to discuss the pathogenethisi of sinomenine on invasion and metastasis by the study of MMP-9, CD44and nm23-H1expression.Methods:Through the method of culture HepG2cells in vitro, choose the growing period of cells, deal with HepG2cells by the different concentration of sinomenine(0.125、0.25、0.5、0.625、1.25、2.5mmol/L)and5-FU (0.1mmol/L) Detect the cell proliferation by MMT.Detect the effects of cell adhesion,invasion and migration by Transwell and Matrigel.Western blot will be used to detect the expression of MMP-9,CD44and nm23-Hl.Results:(1) The Sinomenine showed the inhibition of cell proliferation by deal with HepG2cell after48,72hours.(2) Sinomenine could significantly inhibite the adhesion, invasion, migration ability of HepG2.(3) Sinomenine inhibited the expression of MMP-9, CD44, and increased the expression of nm23-H1in HepG2. Conclusion:(1) Sinomenine can directly inhibit human liver cancer cells’ability of invasion, metastasis and proliferation in vitro.(2) MMP-9, CD44, and nm23-H1may be implicated in the invasion and metastasis inhibition by Sinomenine, which may be carry through the intervention of extracellular matrix.