Research Of Evodiamine On Proliferation, Invasion And Metastasis Of Human Hepatocellular Carcinoma HepG2 Cell

Objective: To study the mechanism and effects of Evodiamine on proliferation, invasion and metastasis of human hepatocellular carcinoma Hep G2 cell. Methods: Hep G2 cells were treated with 5.0,12.5,25.0,50.0,100.0μmol/L of Evodiamine, the cell proliferation was assayed by using MTT method. The invasion and metastasis ability was learned by transwell assay.The ability of cicatrization was analyzed by scratch wounds healing. Colony-forming was determined by Colony formation assay. The Evodiamine effected expression level of MMP-9,PCNA,ERK1/2, P-ERK1/2 in Hep G2 cells was assessed by Western blot. Results: The proliferation inhibit rate of Evodiamine treated groups were higer than control group. The proliferation of Hep G2 cell could be significantly inhibited within limited duration and dosage(p<0.05).Hep G2 cells were treated by 0, 5.0, 25.0, 50.0 μmol/L of Evodiamine,the number of invasion cells were 97.6±6.3, 32.3±1.3, 22.3±2.5, 10.6±3.7 and 106.3±3.2, 57.7±3.2, 26.8±1.6, 15.4±3.9 of metastasis cells respectively(P<0.05). Evodiamine could significantly inhibit cicatrization rate of Hep G2 cell compared with control group,the cicatrization rate of 24,48 h were(23.31±1.03)%,(10.37±1.06)%,(7.92±1.40)% and(13.86±5.57)%,(1.78±3.08)%,0±0%(p<0.05) respectively.The inhibition of Evodiamine to Hep G2 cell colony- forming ability is significantly. The Colony-forming numbers were 147.0±12.1, 94.3±4.0, 0, respectively(P<0.05). The expression level of MMP-9 and PCNA which were(0.53±0.020),(0.35±0.020),(0.21±0.015)and(0.76±0.032),(0.66±0.01),(0.59±0.015), were down- regulating(p<0.05).The expression level of MMP-9 and PCNA which were(0.53±0.020),(0.35±0.020),(0.21±0.015)and(0.76±0.032),(0.66±0.01),(0.59±0.015), were down- regulating(p<0.05)It suggested the expression of P-ERK1/2 were down-regulating. Conclusion: The result of our study suggested that Evodiamine can inhibit the proliferation, invasion, metastasis and colony-forming ability of human hepatocellular carcinoma Hep G2 cells and the possible mechanism may be down-regulation of MMP-9, PCNA, P-ERK 1/2 protein.