| Objective: Radioactive Cerebral Edema (radioactive cerebral edema, RCE)is due to abnormally high levels of brain tissue water content of brain tumorpatients after gamma knife, X knife, and cobalt-60radiation treatment of braindiseases, the resulting radiation zone vice reaction. A higher incidence ofserious impact on the quality of life of radiotherapy in patients with braintumors. RCE of the pathogenesis is still so far is still not particularly clear, butmore recognized by the cytotoxic brain edema doctrine. Water channel proteins(Aquaporins, AQP) is an intrinsic membrane proteins specialized in channelingwater through the formation of membrane channel, so far, has been found inmammals,13members of his family (AQP0to AQP12). Which AQP4abundantly expressed in the mammalian central nervous system glial cells.Now widely recognized that the response of cerebral edema in the radiotherapyprocess, given methylprednisolone and other glucocorticoids can be eased tosome extent, the brain edema. This study was to use radioactive cerebral edemain rats model to study the application after the methylprednisolone treatment,the content of AQP4in the target site in brain tissue and brain tissue moisturecontent changes, the regulation of relations between the clear methylpre-dnisolone and AQP4, for clinical treatment to provide a theoretical basis.Methods: Healthy adult Wistar rats were randomly divided into themethylprednisolone group (n=25), saline control group (n=25) andsham-irradiated group (n=25). Gamma-ray irradiation methylpredniso-lonegroup with the control group. Methylprednisolone group and the saline controlgroup after administration of12h,1d,2d,3d,7d, respectively, divided into five subgroups, respectively, to line the target brain tissue moisture detection,AQP4mRNA detection and AQP4immunohistochemistry detection.Results:1A target brain tissue moisture content of the gamma-rayirradiation in rat brain edema, and reach a peak in the first two days, and thendecreased gradually. By gamma irradiation target brain tissue moisture contentin the saline control group was significantly higher than that of the shamirradiation group (P <0.05). Methylprednisolone treatment group with thecontrol group at each time point of brain tissue water content was less than thecontrol group (P <0.05).2Table of the target brain tissue of AQP4-mRNA Gama ray irradiation ofAQP4-mRNA in the rat brain tissue in two days reached a peak, then graduallydecreased, and the moisture content of brain tissue changes. Target brain tissueof normal saline control group of AQP4-mRNA was significantly higher thanthat of the sham exposure group (P <0.05). Methylprednisolone treatmentgroup with the control group at each time point brain tissue organization ofAQP4-mRNA levels were lower than the control group (P <0.05).3The target brain tissue AQP4immunehistochemistry analysis of thegamma-ray irradiation of AQP4in the brain tissue of rats target the minimumgray value in the first two days, and then rise slowly. Target brain tissue ofnormal saline control group the AQP4gray value is significantly lower than thesham irradiation group (P <0.05). Methylprednisolone treatment group with thecontrol group at each time point brain tissue AQP4gray-scale values werehigher than the control group (P <0.05).Conclusion:1The change is related to radioactive cerebral edema in rat target braintissue moisture content of AQP4-mRNA. 2Methylprednisolone treatment of radioactive cerebral edema in rats, cantarget brain tissue AQP4mRNA content,decreased cerebral edema. |
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