The Expression And Significance Of Beclin-1and Bmi-1in Adenomysis

Adenomyosis is a common gynecological disease, the adenomyosis typical clinicalmanifestations are menstrual disorders、progressive dysmenorrhea、infertility etc，whichseriously affect people’s quality of life,and the conservative treatment of the disease is noteffective.Medical workers try their best to investigate the best treatment according to thepathogensis of adenomyosis.But the adenomysis pathogenesis is still unclear.Thepathogenesis of adenomyosis from the programmed cell death has become one of the hot inthe pharmaceutical sector.Programmed cell death include apoptosis and autophagic cell death.Both of them aredeath program which are controled by different related genes.In recent years the study fromapoptosis angle showed that the occurrence of adenomyosis was regulated by some apoptoticgenes. Autophagy is different from apoptosis.The process of autophagy does not depend onthe participation of caspase.However autophagy and apoptosis play a synergistic role in themaintenance of intracellular homeostasia.Autophagy-related genes are known as autophagyrelated genes.Beclin-1is mammalian specific gene involved in autophagy,and is called asautophagy-related tumor suppressor gene.Beclin-1protein is an important converging pointof autophagy and apoptosis.Beclin-1not only can promote autophagy,but also promoteappoptosis.Bmi-1widely recognized as a proto-oncogene is a transcription inhibitorbelonging to the polycomb group genes family. This class protion can block cell cyclerelated protions p16and p19(human p14) INK4a-ARF to boost cell proliferation、inhibit cellapoptosis.At present there is more researchment of Beclin-1and Bmi-1in cancers. Theexpression of Beclin-1and Bmi-1in adenomyosis little has been reported at home andabroad.Materials and methods:This experiment using immunohistochemistry research the expression of Beclin-l andBmi-1in the38cases of adenomyosis in eutopic, ectopic lesions and20cases of normalendometrial tissue. According to the organization pathological results the adenomyosis groupand the control group are both divided into proliferation period group and secretory periodgroup. All cases ruled out gynecological endocrine diseases、autoimmune deseases、 neoplastic diseases and pelvic inflammatory diseases, all the patient’s menstrual cycle is stilregular,and in three months before were not used hormonal contraception. The ImagePro6.0professional image analysis system measure the density of the average light express positivevalue (MOD). We compare the expression levels of two factors in adenomyosis in eutopic,ectopic endometrium and normal endometrium, and analyze the lining pertinence betweenexpression levels of two factors in the same position in the organization.This experimentdatas use statistical software SPSS17.0to T-test, P <0.05for the difference is statisticallysignificant, the correlation uses the Spearman rank analysis.Result:The results suggested that the expression of Beclin-l in the eutopic lesion ofadenomyosis was lower than the expression in ectopic (P <0.05), the expression in ectopicendometrium significantly below normal endometrium of the control group (P <0.05);compared the Beclin-l expressions in the adenomyosis group proliferation period andsecretory period, the difference was not statistically significant (P>0.05). The expression ofBmi-1in the eutopic lesions is higher than the expressions in ectopic endometrium andnormal endometrium (P <0.05), compared the Bmi-l expressions in the adenomyosis groupproliferation period and secretory period, the difference was not statistically significant (P>0.05). However there is not a positive correlation between the expression of Beclin-l andBmi-1in the adenomyosis（P>0.05）. Beclin-l and Bmi-1play different roles in thepathogenesis of adenomyosis. From the roles of cells autophagy and cell apoptosis in theadenomyosis further studying the treatment of adenomyosis may have a new breakthrough.Conclusion:1. Low expression of Beclin-l and high expression of Bmi-l might be involved in thepathogenesis of adenomyosis;2. The expression of Beclin-l and Bmi-l is unrelated to the menstrual cycle in differentperiods;3. Enhancing cell autophagy and promoting cell apoptosis may provide a new thinkingfor clinical treatment of adenomyosis.