The Expression Of SPAG9 In Adenomyosis And Its Clinical Significance

[Objective] Adenomyosis is generally believed that adenomyosis develops as a result of downgrowth and invagination of the basalis endometrium into the myometrium. The pathogenesis of adenomyosis might be correlated with the factors of immune, vascular formation, neurophysin receptor, apoptosis and heredity. However, the exact mechamisms of adenomyosis are not yet very clear. Sperm associated antigen 9 (SPAG9), is a new member of the caner testis antigen family, known to play a major role in cell growth, proliferation, apoptosis, the function of immune cells, embryogenesis and tumor development. It is not understood that if the expression of SPAG9 is related to adenomyosis. We investigate the expressions of SPAG9 in adenomyosis tissues to learn its role in the development of adenomyosis, these studies will be very important to the clinical treatment of adenomyosis.[Materials and Methods] Taking the ectopic foci of adenomyosis, adenomyosis and normal eutopic endometrial tissues from clinical speciments as the research object. The expressions of SPAG9 protein in the ectopic lesions with myometrium of adenomyosis group (20 cases), the eutopic endometrium group(20 cases), and the normal endometrium group(20 cases) were detected by SP immunohistochemistry, and were compared with eachother.[Results] SPAG9 could express either in ectopic or eutopic endometrium with adenomyosis. SPAG9 expressions in ectopic, eutopic endometrium with adenomyosis were both significantly higher than that of control group (P<0.01); No statistically significant difference was found between in ectopic endometrium and eutopic endometrium (P>0.05). The expression of SPAG9 in adenomyosis ectopic and eutopic endometrium was significantly higher than normal endometrium (P<0.01). There was no significant difference between ectopic endometrium and eutopic endometrium in the observation group (P>0.05). There was no or a few SPAG9 expression in the stromal cells. The difference between proliferative phase endometrium and secretory phase endometrium in every group was not significant (P>0.05). SPAG9 expressions in the three cases of the observation group accepting hormone treatment 3 months before the experiment (2 cases placed mirena,1 case took gestrinone and mifepristone) was lower than the other 17 non-users (P< 0.05).[Conclusion] SPAG9 in adenomyosis lesions showed high expression, which may be one of the mechanisms of the occurrence and development process. It also bring a new target for the diagnosis, detection and treatment of adenomyosis.