Autofluorescence Spectroscopy Of Ductal Lavage Fluid

The development of laser technology and spectroscopy detectiontechnology results in laser spectroscopy of high resolution and high sensitivitywhich makes it available in the biomedical field and become an important meanof clinical diagnosis and monitoring. This article aims to explore the clinicalvalue of autofluorescence spectroscopy of ductal lavage fluid as well as theautofluorescence spectral features of ductal lavage fluid. This article also aimsto work out whether ductal lavage fluid can be used as indicators for earlycancer screening,and to provide additional clinical evidence for the earlydiagnosis of breast disease by fluorescence spectroscopy.Methods:A total of300patients were female, aged26-58years old,35years old as a mean age, all from Jilin University Sino-Japanese FriendshipHospital outpatient and inpatient department.210samples of ductal lavagefluid were collected from patients under fiberoptic ductoscopy for study group.Treat60cases of breast-feeding women in good health as a normal controlgroup whichever offer some of their fresh milk. In addition, select breast cancerpatients confirmed by the postoperative paraffin pathology a total of30cases,and get solid tissues. All of the above specimens were observed in fluorescencespectroscopy detection and analysis system.Results:In this preliminary study, fluorescence spectroscopy of ductallavage fluid and solid tissues was carried out. We found that with405nmexcitation, the main peak of normal solid tissues located at472nm, theremaining two sub-peaks at442nm and506nm. While the main peak ofcancerous solid tissues located at470nm, and only had a secondary peak at444nm. In addition, both peak intensity and peak intensity ratio were alsodifferent. The ratio I442/I470(the secondary peak intensity around442nm,and the peak intensity around470nm)were0.77for normal tissues and0.80forcancerous tissues. As for spectrum images of normal and cancerous solidtissues with670nm excitation, the curves were relavively flat and did not showthe characteristic peaks. Fluorescence spectroscopy showed that, fluorescencespectra of ductal lavage fluid had its own characteristics with405nm excitation,such as the peak located at504nm, and full width at half maximum of the peakwas100nm. By contrast, the trend of the spectral curve of the normal groupwas flat and did not show significant spectral features. The area under the twocurves were processed by statistical analysis, and the result was p <0.05,showing statistically significant differences. Similar to solid tissues, spectrumof the study group and the normal control group showed no significantdifferences with670nm excitation. By comparison of the spectral parametersderived from solid tissues, the spectrum of breast ductal lavage fluid showed itsown characteristics.Conclusion:Ductal lavage fluid showed characteristic autofluorescencespectroscopy. And as an early detection of breast diseases,ductal lavage fluidshowed its potential clinical value.