| With the development of the times, today’s social rhythm accelerated, lifestress, and for prenatal and postnatal care more seriously. According to relatedstatistics, about15%of couples married for one year can not be pregnant andwill seek treatment. There are less than5%of patients can only be forced toaccept the outcome of the barren. Now known to have many causes can lead toinfertility, reduced sperm count is one of the reasons which can not be ignored,and accounts for approximately10-15%of the proportion in which Ychromosome microdeletions.Y chromosome microdeletions and male infertilityare closely related, the highest incidence of in asthenospermia patients. Themost common is that the Y chromosome AZFc deletion, accounting for65%-70%. Recommendations for patients with severe spermatogenicdysfunction (sperm concentration <5×106/ml), the detection of line Yq genedeletion. This article subjects the total number of cases to804cases, improvethe treatment information,804cases of initial filter criteria are men withazoospermia or severe oligozoospermia, the object of study information arefrom November2009to February2012, Jilin University a hospital, the SecondHospital of Jilin University, Sino-Japanese Friendship Hospital urinary maleoutpatient and Jilin Institute of genitourinary,40cases have been successfulfertility male as a control group, analysis of primary azoospermia and severeoligozoospermia patients on the Y chromosome sperm gene (azoospermiafactor, AZF), explore, and reproductive hormone abnormalities, karyotypeabnormal relationship between and Y chromosome microdeletions, such asvaricocele, cryptorchidism, reproductive system diseases relevance. Objective: This study aims to research the primary causes of azoospermiaand severe oligozoospermia in patients with the relationship between these twogroups of patients with varying degrees of spermatogenesis disorders and Ychromosome AZF microdeletions, such as literature and information above, andY chromosome AZF gene microdeletion and several childbirth-related diseasessuch as varicocele, etc.Methods: STS loci multiplex PCR amplification assay of804studied theY chromosome AZF gene microdeletion status, summary semen physical andchemical analysis, semen cytology, seminal plasma biochemical assays, serumreproductive hormone testing, optical microscopy downstreamchromosomekaryotype analysis, testicular fine needle aspiration cytology of thesuction check the results of analysis by the SPSS software to analyze the data.Results:804cases selected for study when the Central Plains azoospermiapatients accounted for584cases, and severe oligozoospermia cases to220cases of Y chromosome microdeletions in the proportion of11.99%and9.09%respectively, the total deletion rate was11.19%. Detected in the overall studyresults were detected for the lack of AZFa District2cases, the deletion of thisregion was2.22%; the the AZFb District deletion were detected in17cases, thedeletion of this region rate of18.89%; of AZFc District deletion were detectedin84cases, the miss rate of this area up to94.44%; AZFd District deletionwere detected in64cases, this area is second only to c area to reach71.11%.804patients with Klinefelter syndrome (karyotype47, XXY)57cases,accounting for7.09%overall; trisomy21syndrome (karyotype47, XY,+21)28cases,3.48%of the total; autosomal inversion and a total of29cases of ectopic,accounting for3.6%of the total,4of the form inversion and ectopic type listedseparately; chimera of two cases, the karyotype were46, XY [34]/45, of X [6],0.25%of the total; increase in Y chromatin, which is a big Y chromosome1cases; Y chromatin reduction, the small Y chromosome of two cases. The overall object of study is divided into groups of azoospermia and oligozoospe-rmia group, respectively, and then with or without microdeletions divisiontesticular volume difference analysis, statistical analysis, the two groups therewere no differences. Study were detected90cases of the existence of Ychromosome AZF microdeletions, prolactin levels were detected in35cases;luteinizing hormone levels were detected in four cases; follicular estrogenhormone levels were detected in29cases; testosterone decreased detection of15cases; estradiol levels were detected in21cases, the object of study towhether the grouping of the Y chromosome microdeletions of the hormonebetween the two groups showed no significant difference (P>0.05). Selectedvaricocele patients in the study accounted for320cases, the Y chromosomeAZF microdeletions were detected in54cases, the missing ratio of16.9%;azoospermia varicocele accounted for484cases, the Y-chromosomemicrodeletions were found to out of75cases, the missing ratio of15.5%. Thetwo groups were compared, confirmed that the Y chromosome microdeletionsin the incidence of no difference.Conclusion: Y-chromosome microdeletions cause of male infertility,among the different types of Y chromosome microdeletions of AZFc+d, theincidence of missing class missing performance was a trend of diversity,according to sievethe check-bit distribution, the highest incidence of DAZ generegion, sY254and sY255missing. Therefore, we recommend the azoospermic,less severe Y chromosome microdeletions in patients with azoospermia andunexplained idiopathic infertility patients should undergo testing to clarify thecause. The results of this study, no azoospermia and severe oligozoospermia inpatients with chromosomal abnormalities was15.05%, higher than the Ychromosome microdeletions in the incidence of still male infertility the mostimportant genetic factors. |
PDF Full Text Request