Effects Of Insulin On Osseointegration In Osseous Tissue Around Implant Of Type2Diabetic Rats

Objective:To establish a type2diabetic rat model and insert implants, study and compare the peri-implant bone binding and TGF-β1expression level on normal rats, diabetic rats with or without insulin intervention,to clarify the relationship between TGF-β1and the peri-implant bone and also to evaluate the function of insulin for Osseointegration around the implant and furthermore to provide experimental data of clinical treatment for the repair of implant of diabetic patients.Methods:1Set up type Ⅱ diabetic rat model:randomly dividing308weeks old male SD rats were into3groups:normal implant group (TO), diabetic implant group (T1) and diabetic implant with insulin intervention group (T2). T1and T2were treated with high fat and sugar diet to trigger insulin resistant, low dose of STZ injection then created type Ⅱ diabetic rat model.2To establish tibial implant model:Titanium implants were inserted into the right tibia near the epiphseal end of all rats groups,12weeks after implantation, rats were killed and taken the right tibia.3Osseointegration around the implant was analyzed using histology and radiographic methods. Expression of TFG-β1in bone tissue around implant was detected using immunohistochemical methods, and further analysis was done on effects of insulin on Osseointegration in osseous tissue around implant of type2diabetic ratsResults:1Rats in general:diabetic rats increased blood glucose, back hair sparse dull, polydipsia and polyuria, weight loss was obvious, less lively; after insulin treatment, blood glucose reduced, significant improvement in various states, however still less well than normal rats.2Implant healing:no loosening or loss of the implant, surrounding soft tissue healing well. X ray indicated that diabetic rats had thinner peri-implant cortical bone, cancellous bone in the trabecular bone density reduced, two of the diabetic rats showed various degree of bone resorption; situation improved after insulin treatment, however less well than normal rats.3The expression level of TFG-β1in bone tissue around implant:TO showed the highest expression level with most intense stain, subsequently T2showed less expression, T1showed lowest expression among the all, lightly stained.Conclusion:1Using insulin to control blood glucose can significantly improve the bone and bone mass of the peri-implant bone, make the peri-implant funnel type bone resorption decreased.The absorption of cortical bone reduced and. trabecular bone density increased.2Type Ⅱ diabetes decreased the expression of TFG-β1in the bone tissue around the implant whereas insulin can increase the expression of TFG-β1and strengthen the implant osseointegration.