The Protective Effects Of Recombinant Human Interleukin10on Lung Ischemia-reperfusion Injury In Rats

ObjectiveTo evaluate the protective effects of recombinant human interleukin10on lung ischemia-reperfusion injury in rats and its possible mechanisms.MethodsRat models of lung ischemia-reperfusion were established by clamping of the left pulmonary arteries, left pulmonary veins and left main bronchus.72healthy Wistar rats were randomly divided into three groups:surgical control group(group Control),ischemia-reperfusion group(group IR) and recombinant human interleukin10pretreated group(group rhIL-10). Every group included24rats(8rats at45min ischemia、60min and120min reperfusion respectively).Rats were sacrificed at relative time point collecting the plasma and lung sample. The plasma contents of malondialdehyde(MDA) and TNF-a were determined. Lung pathology were examined.ResultsThe plasma content of MDA was not different statistically among three groups after45min of ischemia (P>0.05).Both group IR and group rhIL-10showed significantly increased MDA content compared with group Control, while group rhIL-10lower than group IR after60min of reperfusion and120min of reperfusion (P<0.05).The plasma content of TNF-a in group IR was increasing significantly compared with group Control after45min of ischemia (P<0.05).The content of TNF-a in group rhIL-10was not different statistically compared with group Control (P>0.05).Both group IR and group rhIL-10showed significantly increased TNF-α content compared with group Control while group rhIL-10lower than group IR after60min of reperfusion and120min of reperfusion(P<0.05). Pathological examination showed severe polymorphonuclear leukocytes infiltration with bleeding. Group IR was worse than group rhIL-10on the pathological examination. ConclusionsPretreatment with rhIL-10protected the lungs against ischemia-reperfusion injury possibly by suppressing PMN infiltration,reducing production of oxygen radicals,inhabiting reacting of TNF-α.