| Objective:The purpose of the present study was to investigate the protective effect of Ginkgoprescription on kedney ischemia reperfusion injury and its mechanism, and to providescientific basis for the development of new drugs.Methods:40rabbits were treated with3%sodium pentobarbital (30mg/kg) by intraperitonealinjection of anesthesia,back croos fixed on the operation table,abdominal medianincision,separstion of the right kidney and resection,free exposure left kidney and left renalpedicle in rabbits,contron group without occlusion of left renal and left pedicle,directabdomial closure;ischemia rabbits clamping the renal pedicle occlusion of the renal bloodflow of abdominal closure;reperfusion group and treatment group rabbits were clipped renalpedicle occlusion of renal blood flow in30min reperfusion30min, then abdominal closure.Allrabbits were sacrificed after reperfusion for30min and renal preservation for.The treatmentgroup were treated with Ginkgo biloba preparation gavage(10ml/kg),once a day,administeredcontinuously for4weeks,the control group,ischemia group and reperfusion group are in thesame amount of normal saline lavage. The rabbit in the reperfusion time open take blood fordetection of serum BUN,Cr.At the same time take renal tissue with10%formaldehydesolution fixed24h,ethanol dehydration,paraffin embedding,sectioning,hematoxylin andeosin(HE) staining was observed under the light microscope, the pathologic changes ofkidney.Using thiobarbituric acid in the detection of renal tissue MDA content;xanthineoxidase assay for the detection of renal tissue in SOD,GSH-Px activity.Byimmunohistochemical SP method for the detection of renal tissue intercellular adhesionmolecule-1and P-selecin expression.Results:1.Reperfusion group compared with the control group, the MDA content increasedsignificantly, SOD, GSH-Px activity was significantly reduced, there is significant difference;Ginkgo biloba treatment group decreased the content of MDA, SOD, GSH-Px activityincreased, and reperfusion group compared with the significance of difference;2.Under an optical microscope, a control group of tublar neatly arranged, renal interstitialwithout obvious hyperemia, edema; renal ischemia group30min tubular derangement is sparse, and tubule were dilatation and renal interstitial hyperemia, edema; renal ischemiareperfusion group30min30min tubular irregularly arranged, part of the epithelial celldegeneration, kidey interstitial hyperemia edema significantly heavier; Ginkgo bilobatreatment of renal tubular derangement is reduced apparently,epithelial cells without apparentdegeneration, renal interstitial hyperemia is reduced.3.Reperfusion group serum BUN and Cr increased significantly, compared with thecontrol group was statistically significant, illustrate the model of successful preparation.Ginkgo biloba preparation group can obviously reduce the acute ischemia reperfusion injuryin rabbits under BUN and Cr levels, and reperfusion group compared with statisticalsignificance.4.In ischemia reperfusion group of renal tissue intercellular adhesion molecule ICAM-1,P-selectin elevated levels of expression, the Ginkgo biloba preparation treatment after renaltissue intercellular adhesion molecule ICAM-1, P-selectin expression was inhibited, andreperfusion group compared with statistical significance.Conclusions:1.Ginkgo biloba preparations significantly decreased serum BUN and Cr levels, andmarked improvement in renal interstitial edema and renal tubular epithelial cell function,thereby protecting the ischemia reperfusion kidney tissue.2.Ginkgo biloba preparation can significantly decrease the content of MDA in renal tissueafter ischemia-reperfusion injury, increased SOD, GSH-PX activity, tips it has obviousscavenging oxygen free radicals protect the kidney tissue.3.Intercellular adhesion molecule ICAM-1, P-selectin involvement in renal ischemiareperfusion injury mechanism, ginkgo extract compound can obviously reduce the expression,on renal ischemia reperfusion injury in rabbits with protection function. |
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