| BackgroundWith age, skin laxity, the contours of change and the formation of wrinkles are creating difficulties to many people, and how to prevent and delay the aging of the skin has become one of the focus. In recent years, skin cosmetology has become a tendency, for example minimally invasive, non-invasive operation techniques. People seek treatment so that the dermis updates, organizational structure can become better, and make epidermis return to normal. Radio frequency Skin refirming is a non-invasive, non-ablative medical cosmetic technology in recent year.Radio frequency high frequency is electromagnetic waves with the change of a high frequency alternating current, which exerts its biological effects through the selective heating effect. Richard et al reported that radio frequency technology can smooth wrinkle, improve skin laxity, aging, improve skin texture and other effects. Radio frequency technology had been carried out more comprehensive exposition by Hong Liu et al. Although the technology has been widely used in clinical, there is rarely report about the radio frequency mechanism which is still not very clear, limiting the further development and use of radio frequency smoothing wrinkle technology. This study was designed dynamic observation of radio frequency technology on cavia cobaya skin tissue morphology and collagen content, to further explore the radio frequency mechanism.ObjectiveAfter monopole radio frequency action eavia eobaya skin, to evaluate the histologieal ehange of skin with HE stains; using Maria Victoria blue ponceau S double stains to evaluate the skin collagen histologieal ehange; Skin histological microstructure were observed by transmission electron microscope;to quantitative analysis of hydroxyl Prolineeontent. Confirm meehanism of action for radio frequency on human skin. MethodsSeleeted40eavia cobayas.Each of back skin was divided into radiofrequency areas and non-radiofrequency areas randomly. Using monopole radio frequency act on cavia cobaya back skin five times and one time a week. Power was60W, and accumulative energy was10KJ. Skin samples after radio frequency were respectively got after radio frequency instantly, one month, two months and three months. Sizes were8mm x5mm x3mm.1. TO evaluatethe histologieal ehange of skin with HE stains; to evaluate the collagen fiber ehange with Maria Victoria blue ponceau S double stains and compares with non-RF.2. It produces a transmission electron microscopy specimen using1mm x3-4mm fresh specimens, then observes the ultrastructural changes of collagen fibers and fibroblasts in transmission electron microscopy and compares with non-RF.3. The content of hydroxyproline in the skin of each group of RF area and non-RF area were detected using sample alkaline hydrolysis and quantitative detection, and were statistically analyzed by repeated measures analysis of variance.Results1. Observed by light microscope and compared with the control plot, there were no significant changes in cavia cobaya epidermal thickness after the RF current role. Blood capillary organization and the structure of hair follicle were integrated and without any damage, and some of the endothelial cell swelled. In addition, the RF instantly had loose arrangement of the dermis layer of collagen, a slight disturbance, a small number of curls and united into a group. RF after one month dermal collagen arranged rules. RF after two months or three months the dermis layer of collagen staining continue to be deeper, the arrangement of collagen fibers were tidy, compact dense structure, significantly stronger than the control area, and visible increasing in elastic fibers and fibroblasts.2. Observed by transmission electron microscopy, non-RF area and radio frequency (RF) areas were visible fibroblast nuclear membrane and the structure of membrane integrated.Compared with non-RF area there are slight disorders of collagen fibers in the immediate group of RF area. Fibroblast endoplasmic reticulum was dilated. Part of the mitochondrial structure was fuzzy. The radio frequency district among one month to three months, the arrangement of collagen fibers were gradually dense, fibroblasts gradually increased, the endoplasmic reticulum had mild expansion, and the structure of mitochondria was gradually clear and swelling and distension reduced.3. Compared with non-RF area there is no significant difference in content of hydroxyproline. The content of hydroxyproline in RF skin was consistently higher than non-RF area from one month to three months with a significant difference (p<0001).Conclusions1. The role of radio frequency (RF) to cavia cobaya skin can stimulate the increase of dermal collagen content and the arrangement of collagen fibers were gradually dense.2. The therapeutic effect of radio frequency role on cavia cobaya skin has a certain degree of timeliness which at least maintains three months. |
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