| Objective:To investigate the effect of Dry Cod Ckin Collagen Oligopeptide(DCCO)on skin wound healing in rats.Methods:(1)Choose 48 healthy male(Sprague Dawley,SD)rats,weighing between 210g and randomly divided into 4 groups:control group(NS group,n=12),oral DCCO group(K group,n=12),external DCCO group(group W,n=12),oral combined external use group(group L,n=12).(2)Make two 1.0cm×1.0cm wounds on the back and neck of the rat,and perform the following operations after the model is completed:NS group(0.9%saline intragastrically,clean gauze applied to the wound),K Group(900mg.kg-1.bw-1 DCCO solution gavage,external application of clean gauze at the wound),Group W(0.9%saline gavage,wound covered with GSCP fully moistened gauze),Group L(concentration 900mg.kg-1.bw-1 DCCO solution was infused intragastrically,and the wound was covered with wet gauze fully saturated with DCCO solution).From the next day after surgery,every day at 10 am on time.(3)Six rats were randomly selected from each experimental group at 7 and 14 days after modeling,and the full-thickness skin tissue of 4.0 mm on the right edge of the wound was taken and fixed in 10%formalin solution.Paraffin cuts were used for HE staining analysis and collagen fiber staining,and the skin at 4.0 mm on the left edge of the wound was used for the determination of hydroxyproline content.The wound healing of each group of rats was recorded on days 2,4,6,8,10,12,and 14 after modeling,and the wound healing rate was calculated.Results:(1)Skin wound healing rate of rats:The skin wound healing rate of rats in groups L,W,and K was significantly different from that of the NS group from the second day after modeling(P<0.05).The wound healing rate on the second day after modeling(21.96%in group K,19.3%in group W,and 24.67%in group L)was extremely significantly(P<0.01)higher than that in the control group(9.53%).On the 14th day,the wound healing rate(95.11%in group K,95.52%in group W,and 96.00%in group L)was extremely significantly(P<0.01)higher than that in the control group(89.73%),and the wounds in the DCCO treatment group had reached the complete healing criteria.2)Histopathological observation revealed that there were fewer inflammatory cells and higher fibroblast content in the skin wound tissue of rats in groups L,W,and K compared with the control group.3)Collagen fiber staining revealed that the percentage of collagen fiber content on the 7th day after modeling in groups L,W,and K(45.89%in group K,46.57%in group W,and 53.82%in group L)was extremely significantly(P<0.01)higher than that in the control group(33.11%).On the 14th day after modeling,the percentage of collagen fiber content in the skin wound tissue of rats(56.32%in group K and 66.74%in group L)was extremely significantly(P<0.01)higher than that in the control group(49.87%),and the collagen fiber content in the skin wound tissue of rats in group L was significantly(P<0.05)higher than that in groups K and W.(Fig.4)The determination of hydroxyproline content revealed that the content of hydroxyproline in the skin wound tissue of rats in groups L,W,and K on days 7 and 14 after modeling was significantly(P<0.05)higher than that in the control group.5),both oral and topical administration of DCCO could effectively promote the healing of skin wounds in rats,and the combined administration was more effective.Conclusion:(1)DCCO can effectively improve the skin wound healing rate of rats,reduce the skin wound inflammatory response,promote the accumulation of collagen around the skin,thereby promoting skin wound healing.2),both oral and topical administration of collagen oligopeptides from dried cod skin could effectively promote the healing of skin wounds in rats,and the combined administration was more effective. |
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