Expression Of HIC1、P53and OVCA1in Ovarian Cancer And Its Clinicopathological Significance

Background and objective:Ovarian cancer is one of the the most common gynecological malignant tumor and is no evident symptoms in early stage. This type of cancer is often discovered at late stages and is short of effective therapies, threatening the well being of the female. The discovery of these molecular events has proven to be important for ovarian cancer. In recent years, studies have established that chromosome17is a hotspol for chromosome aberrations in ovarian cancer Candidate tumor suppressors located on these chromosomes include HIC1、P53and OVCA1.In this study we focus on the expression of P53、HIC1and OVCA1proteins in ovarian epithelial tumors and investigate the relationships between this expression and the clinicopathological features of ovarian epithelial tumors.Methods:For this study,99cases of surgically resected ovarian tissue were collected from2008to2010, and the routine H&E-stained were reviewed to verify the morphologic diagnosis. We used western blot to detect the expression of P53、HIC1and OVCA1proteins and use immunohistochemistry method to detect P53protein in20cases of normal ovarian tissue,20cases of benign ovarian tumor,20cases of borderline ovarian tumor, and39cases of ovarian cancer. Statistical analysis was performed using the SPSS13.0software.Results1. There was significantly lower level of HIC1protein expression in ovarian cancer than in normal ovarian tissue, benign ovarian tumor, and borderline ovarian tumor(P<0.05); There was significantly lower level of HIC1protein expression in borderline ovarian tumor than in normal ovarian tissue, benign ovarian tumor (P<0.05).2. There was higher level of P53protein expression in ovarian cancer than in borderline ovarian tumor and normal ovarian tissue and benign ovarian tumor(P<0.05).3. The expression of P53in serous cystadenocarcinoma was higher than that in endometrioid carcinoma and mucinous cystadenocarcinoma (P<0.05),and the expression of P53in poorly differentiated and moderately differentiated ovarian cancer tissues was higher than that in well differentiated tissues.4. There was lower level of OVCA1protein expression in ovarian cancer and borderline ovarian tumor than in normal ovarian tissue and benign ovarian tumor (P<0.05).5. The expression of OVCA1in poorly differentiated ovarian cancer was lower than that in well differentiated tissues(P<0.05),and the expression of ovarian cancers in stage III and IV was significantly lower than that in stage Ⅰ and Ⅱ(P<0.05).6. The expression of HICl was negative correlated with that of P53(P<0.01, R=-0.37085) Conclusions1. I here is significant differences among ovarian cancer, normal ovarian tissue, benign ovarian tumor, and borderline ovarian tumor. The lower level expression of HIC1proteins might play a role in the aetiology of ovarian cancer and might correlate with the development of ovarian cancer.2. The higher level expression of P53proteins might be associated with the pathogenesis of ovarian cancer and the development of it. HIC1might cooperated with P53during the process of tumorigenesis.3. These data suggested that the lower level of expression of OVCA1proteins might play a role in the aetiology of borderline ovarian tumor and ovarian cancer and it might correlate with the development of ovarian cancer.