Correlation Of Plasma Microrna-122with Hepatectomy-Induced Liver Injury

Objective:To investigate the correlation of plasma microRNA-122(miR-122) level with hepatectomy-induced liver injury in hepatocellular carcinoma(HCC) patients..Methods:Plasma miR-122was detected in20healthy controls and30patients with HCC by quantitative fluorescent real-time reverse-transcription polymerase chain reaction.Then, plasma miR-122was also detected in30patients with HCC in different times(before operation, the first day and the seventh day after operation) by the same method. Moreover, the miR-122level was compared with clinicopathological parameters of the patients.Results:(1)Plasma from patients with HCC contained higher levels of miR-122than plasma from healthy controls (P<0.05)(2) Plasma miR-122levels in patients with HCC at different times(before operation, the first day and the seventh day after operation)were positive correlated with plasma level of alanine aminotransferase(ALT), Aspartate aminotransferase(AST) and bilirubin (R>0.7,P<0.05),it was negative correlated with plasma prealbumin,it was not correlated with plasma albumin and prothrombin time.(3)Plasma miR-122levels in patients with HCC at the1st day post-operation correlated well with hepatic inflow occlusion, tumor size, liver cirrhosis and HBE e antigen(P<0.05) positive.There was no correlation of miR-122with other clinicopathological parameters including age, sex, blood loss in operation,vessal thrombus, capsul integrity and lpha-fetal protein.Conclusion:Plasma miR-122has potential as a novel and reliable blood marker for hepatectomy-induced liver injury in HCC patients.