Animal Experimental Study Of The Relationship Between Red Pigment And Pathogenicity Of Penicillium Marneffei

Objective preliminary study the relationship between red pigment and pathogencity of Penicillium marneffei.Method15isolates from Guang Xi Bamboo rats isolates and15patients isolates were selected and cultured in SDA liquid25℃for72hours, and then the OD values were acquired, represent the pigment Select the maximum, median, minimum isolates, in which the suspension was injected into normal mice from abdominal. Observe the general status, infection status of internal organs. Mice survival after infection and colony count of liver in mice were calculated. And Six weeks later the remaining mice all executed, also observe the infection status of internal organs, calculate colony count of liver and carries on statistics analysis.Results It is demonstrated that all30isolates could produced red pigment in different levels, while there was no significant difference in statistics between two different sources of PM(P<0.05). There was different infection status of internal organs, survival and colony count of liver in different groups of OD value, and then the higher of the OD value, the more serious of the internal organs infection and the colony count of liver, at the same time the lower of the survival rate.Conclusion The red pigment in25℃which probably involved in the Virulence markers. There was no significant difference of the red pigment between two different sources of PM. There is not significant difference of pathogenicity between PM isolated from bamboo rats and patients.