| Objective: To prove the truth of the the morphology difference among the Penicillium marneffei isolates; To study the extracellular proteinase activity among the Penicillium marneffei isolates; To study the effect factors of proteinase activity. To probe the relationship between the morphology, extracellular proteinase and the pathogenesis on Penicillium marneffei.Methods: 4 isolates of Penicillium marneffei was inoculated to the culture liquid medium, and observe the difference of the morphology. then inoculated to Hb-YCB medium and BSA-YCB medium in vibrator for 9 days, then selected the culture supematant.Used the QuantiCleave proteinase Assay Kit to test the proteinase activity of 4 Penicillium marneffei isolates. The temperature .and pH on this assay was identical. The enzymatic activities of P. marneffei yeast. The whole cell yeast and cell-free supernatants were determined with the semi-quantitative Api-Zym system as described and recommended by the manufacturer. which were then incubated for 4 h at 37℃. The pre-supplied colour reagent was added to determine enzymatic activity.Result: Morphotype MM was similar to the P by colony characteristics andby microscopy. In contrast, morphotype MY and A demonstrated differentfrom those of the P. The method of QuantiCleave proteinase Assay Kit: 9days after inoculation, the supernatant proteinase activity of the MY strain ishigher than that of the others (p<0.01);There is little difference in type ofextracellular proteinase between either the 4 isolates or whole cell yeasts andcell-free supernatants.Conclusion: MY strain is high proteinase activity isolate. Little difference isfound in type of extracellular proteinase among the 4 isolates or between thewhole cell yeasts and cell-free supernatants. it is hypothesized that thisproteinase may be involved in tissue invasion, pathogenesis, and/orconversion to the yeast phase.
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