| Objective:To investigate the expression of embryonic stem cells key transcription factor Nanog in ovarian cancer and cancer stem cells.Methods:Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were performed to detect the expression of Nanog gene in 10 cases of normal ovaries,10 cases of benign ovarian tumors and 60 cases of ovarian cancer. Ovarian caner stem cells were isolated from ovarian cell line SKOV-3 and cultured in serum free medium by nanosphere suspension. Flow cytometry analysis was used to examine the expression of the cell surface marker CD117 in SKOV-3 and ovarian cancer stem cells. The expression of Nanog gene in SKOV-3 and ovarian cancer stem cells were detected by RT-PCR and Western Blot.Results:Nanog gene expression in ovarian cancer was significantly higher than that in normal ovarian tissue and benign ovarian tumors (P<0.05). The Nanog expression was higher in ovarian cancer with poorly differentiated grade or advanced stage than that in well differentiated or early stage.respectively (P<0.05). Ovarian cancer stem cells were isolated, the relative level of Nanog mRNA was 0.6044±0.0368 in SKOV-3 and 0.8736±0.0537 in ovarian tumor stem cell (P<0.05). The relative level of Nanog protein was 0.6364±0.0169 in SKOV-3, and 1.2788±0.0314 in ovarian tumor stem cell (P<0.05).Conclusions:Nanog gene expressed highly in ovarian cancer and SKOV-3, which maybe the antigen marker of ovarian cancer stem cells and could be as novel marker for ovarian cancer. |
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