| Objective:Objective to explore the change of NANOG and SOX2 in the process of disease occurence,development and infiltration process so as to provide opposite theory for diagnosis and therapy of ovarian cancer in gene level.Method:140 cases of paraffin block specimens of ovarian pathology tissue that were incised surgically in Qingdao University Affiliated Hospital during the period of June 2014 to June 2017 were collected,all the specimen were pathologically confirmed postoperatively.Of these,there were 80 cases with ovarian epithelial malignancy,20 cases with ovarian epithelial borderline tumor,20 cases with ovarian tumor,20 cases of normal ovarian tissue.Expression of NANOG and SOX2 in these cases were detected by immunohistochemical method.The laboratory data was statistically processed using SPSS19.0 software,chi-square test was used for numerical data analysis to explore relationship of clinical pathological parameter of the patients with NANOG and SOX2,detected data underwent Spearman rank correlation analysis for laboratory data analysis,and study was conducted on the correlation of expression of NANOG and SOX2 in ovarian epithelial malignancy.Results:1.Positive expression rates of NANOG in normal ovarian tissue,ovarian epithelial benign tumor,borderline tumor and malignancy were 20%,20%,30% and 83.75%.Positive expression rates of NANOG in normal ovarian tissue,ovarian epithelial benign tumor and borderline tumor have no obvious difference(P>0.05),however,compared with epithelial malignancy,all the differences have statistical significance(P<0.05).In ovarian epithelial serosity and mucinous carcinoma,NANOG showed high expression,the difference has no statistical significance(P>0.05).According to FIGO(2014)clinical staging,positive expression rate of NANOG inⅢ∽Ⅳphase ovarian cancer tissue washigher than I ∽Ⅱphase,the difference was of statistical significance(P<0.05).In different degrees of differentiated(highly moderately and lowly differentiated)cancer tissue,positive expression rate of NANOG was increased gradually,i.e.,the lower the differentiation extent,the higher the positive expression rate of NANOG.And difference of comparison on between two-two has statistical significance(P<0.05).Positive expression rate of NANOG in lymph node metastasis group was 93.94%,positive expression rate in non-metastasis of lymph node was 76.60%,the difference is of statistical significance(P<0.05).2.Positive expression rates of SOX2 in normal ovarian tissue,ovarian epithelial benign tumor,borderline tumor and malignancy were respectively 10%,25%,25% and82.5%,Positive expression rates of SOX2 in normal ovarian tissue,ovarian benign and borderline and ovarian epithelial tumor have no obvious difference(P>0.05),however,compared with ovarian epithelial malignancy,all the differences have statistical significance(P<0.05).In ovarian epithelial serosity and mucinous malignancy,SOX2 showed high expression,the difference has no statistical significance(P>0.05).According to FIGO(2014)clinical staging,positive expression rate of SOX2 in I ∽Ⅱ phase ovarian cancer tissue was lower thanⅢ∽Ⅳphase,the difference was of statistical significance(P<0.05).In different degrees of differentiated(highly moderately and lowly differentiated)cancer tissue,positive expression rate of SOX2 was increased gradually,i.e.,the lower the differentiation extent,the higher the positive expression rate of SOX2.Difference of comparison on between two-two has statistical significance(P<0.05).Positive expression rate of SOX2 in lymph node metastasis group was96.97%,positive expression rate in non-metastasis of lymph node was 72.34%,the difference is of statistical significance(P<0.05).3.The expression of NANOG and the expression of SOX2 were positively correlated,correlation coefficient r=0.511,p<0.0001.Conclusion:1.Expression of NANOG and SOX2 in ovarian epithelial cancer tissue was strengthened.2.In ovarian epithelial cancer tissue,expression of NANOG and SOX2 is related with clinical staging,differentiation extent and no-lymph node metastasis,and it is unrelated to pathological type of tumor.3.Expression of NANOG and SOX2 in ovarian epithelial cancer tissue showed positive correlation. |
