Experimental Study On The Effects Of M1and M2Macrophages On The Gastric Tumor SGC-7901

Objective:To discuss the effects of Ml and M2macrophages on the SGC-7901gastric tumor cells in vitro.Methods:Monocytes which were isolated from healthy volunteers peripheral blood by the MACS CD14Microbeads were cultured and induced into two subgroups:granulocyte macrophage colony stimulating factor(GM-CSF)used to obtain Ml macrophages,and macrophage colony stimulating factor(M-CSF)used to obtain M2macrophages.Then their phenotypes were identified by CD16and CD163.These two subgroups and monocytes were cultured with gastric tumor cells by transwell system.The number of living gastric cells was counted and establishing the growth curve.The apoptosis of gastric cells was detected by hoechst kit.The invasiveness of gastric cells was detected by counting living cells.Result:Monocytes which were induced respectively by GM-CSF and M-CSF expressed highlevel CD16(94.87±2.35)(p<0.01)and CD163(95.28±1.87)(p<0.01).The group M2grastic cells grew faster(34.05±1.82)than blank controller group(16.38±1.23)and Ml group(0.88±0.43).The number of cut through group M2grastic cells were larger(289.50±8.78)than blank controller group(192.50±5.39)and Ml group(37.83±3.19)(p<0.01).The apoptosis rate of group Ml grastic cells(83.07±1.71)were higher than blank controller group(2.57±0.99)and M2group(2.47±1.14)(p<0.01).Conclusion:(1)M1macrophages can promote the gastric cells apoptosis,suppress their growth.(2)M2macrophages can promote gastric cells growth and invasion.