Study On Composition Differences Of Fecal Microbiota In Patients With Stomach,colon And Rectal Neoplasms

Research purposes: To analyze and compare the diversity and composition of fecal microbiota between patient respectively with stomach,colon,rectal carcinomas and healthy controls.Providing a reference results of biomarkers which with the potential for monitoring the health of the gastrointestinal tract,and promoting the development of non-invasive screening and monitoring methods for gastrointestinal tumor.Methods: This research is a case-control study.We studied gut microbiota by 16 S r DNA sequencing from fecal samples,which were collected from 56 patients with gastrointestinal cancers and 20 healthy individuals from the outpatient and inpatient department of our hospital from June 2018 to July 2019.Each patient was definitely diagnosed with gastric cancer,colon cancer or rectal cancer by endoscopy and tissue examination.The principles and methods of bioinformatics analysis were used to compare the diversity and composition of fecal microbiota between different patient groups and healthy controls.Results: There are differences between the composition of fecal microbiota from patients with gastrointestinal cancers and healthy controls,which showed a dysbacteriosis: As compared to healthy controls,the abundance of butyric acid-producing bacteria belonged to the family Lachnospiraceae and Ruminococcaceae was reduced in patient groups.Lef Se analysis showed that there was a large number of different species among the groups.Among them,the increased abundance of Lactobacillus is associated with gastric cancer;the increased abundance of Peptostreptococcus,Fusobacterium,Parvimonas,Eggerthella and Megamonas is associated with colorectal cancer.The overall alteration trend of intestinal microbiota in patients with colon cancer was consistent with that in patients with rectal cancer,However,the Bifidobacterium was observed significantly decreased in patients with colon cancer when compared with rectal cancer.Conclusion: This study showed that the composition of fecal microbiota varied with the site of the gastrointestinal tract tumor.The changes of disease-related characteristic microbiota mentioned above in fecal may indicate the impairment of gastrointestinal tract health.Which will provide us some reference for non-invasive screening and monitoring methods for gastrointestinal tract tumor and targeted therapy based on microbial biomarkers in the future.