The Anti-proliferative Effect Of SJAMP On Human Pancreatic Cancer Cell

Objective To investigate the apoptosis-inducing effect and anti-proliferative effect of SJAMP on human pancreatic cancer cell sw1990 in vitro.Methods The proliferation was evaluated by MTT after the cells were incubated with differents concentration and hours of SJAMP. The apoptosis-inducing effect was determined by flow cytometry after the cells were treated with different hours at the dose of 4mg/ml. The cell cyle of sw1990 cells was detected by flow cytometry after the cells incubated with different concentration of SJAMP for 24 hours later.Results Incubated with different concentrations (0mg/ml、4mg/ml、16mg/ml、) of SJAMP, the OD values of grops above-mentioned were 0.78±0.04、0.64±0.01、0.53±0.04 after 24 hours,0.76±0.02、0.42±0.02、0.24±0.03 after 48 hours,0.79±0.02、0.39±0.04、0.24±0.04 after 72 hours. With the increase of concentration and the incubated time the proliferation-inhibiting effect of SJAMP on human pancreatic cancer cell was gradually enhanced, the number of normal cancer cells decreased and the apoptotic rates gradually increase (P<0.01). Treated with 0mg/ml and 4mg/ml of SJAMP, the early apoptotic rate of sw1990 were 3.50±0.34%、11.10±0.75% after 24 hours,3.80±0.36%、21.30±0.91% after 48 hours and 4.40±0.26%、30.80±0.61% after 72 hours. SJAMP can regulate the cell cyle of sw1990 cells. After incubated with different concentrations (0 mg/ml、2 mg/ml、4 mg/ml、8 mg/ml、16 mg/ml、32 mg/ml) of SJAMP, the percentages of cells in G0/G1 stage were 56.49±0.77%、60.38±1.03%、63.79±1.41%、67.38±2.47% 69.43±0.58%and71.57±1.48%.With the increase of the concentration, SJAMP caused cell growth arrest at G0/G1 stage of cell cycle (P<0.01)Conclusions SJAMP can significantly inhibit the cell growth of sw1990 cells. The mechanism of inhibitation might be ralated to the apoptosis-inducing and the regulation of the cell cyle of the sw1990 cells.