Experimental Study On The Effect Of SJAMP On Apoptosis Of Human Hepatocellular Carcinoma Cell Line HepG2 In Vitro

Objective Through studying the apoptosis induced by SJAMP in the hepatocellular carcinoma cell line HepG2 in vitro, finding its mechanism, and analysising the expression of survi vin,bax and Rb in HepG2, To provide the theory foundation and its feasibility that whether it can be used for the chemotherapy of hepatocellular carcinoma.Methods The cells of HepG2 were cultured in vitro and treated with SJAMP at different doses(0.25,0.5,1.0,2.0,4.0mg/ml). MTT was used to observe the inhibitory effects of SJAMP on cell growth, Western Blot was used to detect apoptosis, and to detect the apoptosis related change of expression of protein survivin,bax and RbResults (1)SJAMP produced an obvious time-and-dose-dependent inhibitory effect on the HepG2 cells. (2)MTT identified that SJAMP can induce the apoptosis of HepG2 cells.(3)Western blot showed that SJAMP can induce the apoptosis of HepG2 cells through changing the expression of the protein of survivin and bax(P<0.05), comparing with the control. (4)but SJAMP counts for nothing through Rb protein (P>0.05).Conclusion (1)SJAMP produced obvious inhibitory effects on HepG2 cells and induce-d HepG2 apoptosis. (2)SJAMP can induce the anti-tumor function in the methord of changing the expression of protein surviving and bax. (3)SJAMP has no influence on protein Rb in the HepG2 cell.