The Expression And Clinical Significance Of MicroRNA-192 And MicroRNA-215 In Serum Of Patients With Gastric Cancer

Objective：To study the expression of microRNA-192(miR-192) andmicroRNA-215(miR-215) in serum of patients with gastric cancer and healthycontrols, and to evaluated the association between miR-192 and miR-215expression levels and clinic-pathological characteristics of gastric cancer, andto explore the value of miR-192 and miR-215 in the diagnosis of gastriccancer.Methods: Sera were collected from twenty nine cases of preoperativegastric cancer patients and ten healthy controls. The clinical data of gastriccancer patients were collected. After extracted the total RNA in the serumsamples, expression levels of miR-192 and miR-215 were determined byquantitative reverse transcription polymerase chain reaction (qRT-PCR), andto evaluate the association between miR-192, and miR-215 expressionlevels and clinic-pathological characteristics. The sensitivity and specificity ofdiagnosis for gastric cancer were evaluated by using receiver operatingcharacteristic (ROC) curve and area under the ROC curve (AUC).Results: The expression of miR-192 and miR-215 have an 8.87-fold(P=0.002) and 4.14-fold (P=0.037) increase in the sera of gastric cancerpatients compared with normal controls, respectively. MiR-192 and miR-215expression levels were not significant different between different tumor stage,lymph node metastasis and tumor size. The AUC for miR-192, miR-215, andcombination were 0.833[95%CI(confidence interval):0.793~0.993, P <0.001] ,0.724 (95%CI: 0.555~0.894, P =0.002), and 0.893(95%CI: 0.699~0.966, P=0.037), respectively. The sensitivity for miR-192, miR-215, and combination were 75.9%, 62.1%, 75.9%, and specificity for them were 90.0%, 88.3%,92.1%, respectively.Conclusion:1、Altered expression of miR-192 and miR-215 might be involved in thepathogenesis of gastric cancer.2、MiR-192 and miR-215 expression levels were not significant differentbetween different tumor stage, lymph node metastasis and tumor size. Alteredexpression of miR-192 and miR-215 might be an early event of thepathogenesis of gastric cancer.3、The detection of the microRNAs in serum might be a novel toolfor diagnosis of gastric cancers.