The Basic And Clinical Research Of Perilla Oil And Fenofibrate On Blood Lipid And Vessels

Objective: To compare the effects of perilla oil and fenofibrate on blood lipid andvessels in hyperlipidemia.Methods：(Ⅰ) Basic Research: Forty（8weeks-old，male）Sprague Dawley rats wererandomly and evenly divided into five groups：normal control（normal diet），modelcontro（lhigh-fat diet），fenofibrate group（high-fat diet+100mg/Kg d fenofibrate），perillaoil low-dose treatment group（high-fat diet+0.34g/Kg d perilla oil），perilla oil high dosetreatment group（high-fat diet+2.00g/Kg d perilla oil）。After12weeks of feeding，therats were sacrificed for detection of the levels of lipid、interleukin-6(IL-6)。Wall tolumen area ratio (W/L) of mesenteric artery(3rd grade branch) were assessed afterhematoxylineosin staining。Vascular reactivity to vasoactive substance were recordedusing mesenteric artery rings,which was solated from rats by automatic balancerecording meter。(Ⅱ) Clinical Research:60cases of patients with hyperlipidemia wererandomly divided into two groups，treating with perilla oil soft capsule and fenofibraterespectively for8weeks。Blood lipids ingredients、liver function、brachial-anklepulse wave velocity（baPWV）、ankle brachial index（ABI）、large artery elasticity（C1）、small artery elasticity（C2）were measured in the two groups before and aftertreatment。Results:(Ⅰ) Basic Research:（1）After feeding12weeks，the rat weight of modelcontrol and perilla oil low-dose treatment group were significantly higher than that innormal control （P<0.05），when fenofibrate group and perilla oil high dose treatmentgroup were lower than model control（P<0.05）。（2）Total Cholesterol（TC）、lowdensity lipoprotein cholesterin（LDL-C）、triglycercide（TG）、very lowdensitylipoprotein cholesterol（VLDL-C） and IL-6in model control were significantly higher than normal contro（lP<0.05）；perilla oil low and high-dose treatment all couldreduce Blood Lipid and IL-6，and the effects on LDL-C、TG、VLDL-C exerted thedose-dependent manner（P<0.05）。No significantly difference was founded betweenperilla oil high dose treatment group and fenofibrate group（P>0.05）。（3）In modelcontrol，the W/L was much more higher and the relaxation sensitivity to sodiumnitroprusside was significantly decreased than normal control（P<0.01），perilla oilcould improved on W/L （P<0.05） and relaxation sensitivity to sodiumnitroprusside(P<0.01)by dose-dependent，without significantly difference to normalcontro（lP>0.05）。(Ⅱ)Clinical Research:（1）Triglyceride was significantly decreasedin both of the two groups，and the fenofibrate group was much more remarkable（30.4%VS20.7%，P<0.05）。（2）baPWV、C1and C2were dropped dramatically，without Significantly Differences between perilla oil soft capsule group andfenofibrate group。（3）Two cases(6.7%）of patients showed up mild hepaticdysfunction in fenofibrate group,and recoverded normal after altered to perilla oil softcapsule, d none of patients treated with perilla oil soft capsule had hypohepatia。Conclusion: Perilla oil may ameliorated dyslipidemia，reduced inflammatoryfactor，attenuated vascular hypertrophy,improved the vessels function of the hyperlipidemia，and the curative effets were same to fenofibrate，without hypohepatia。...