Association Of AGT Gene Polymorphisms With Essential Hypertension In Chinese Populations

Objective：Hypertension is the most common chronic disease, as well as one of the main risk factors forcadiovascular diseases. At present, there are about200million hypertension patients in China, withthe possibility that among each ten adults there might be two patients with hypertension. Essentialhypertension (EH) accounted for95%of hypertension patients. Studies have found that essentialhypertension is a common complex disease caused by the interaction of multiple genes andenvironmental factors, though its pathogenesis is not entirely understood. In this study, groupmatched case-control study were used to explore the association between tagSNP polymorphismsof the most researched angiotensinogen gene (AGT) and essential hypertension in Ningbo Hanpopulation and also to investigate the interaction between the AGT gene polymorphisms andenvironmental factors. This study aims to explore the pathogenesis of essential hypertension, andtherefore to provide clues for clinical prevention and treatment of this disease.Methods：1. In this study all the literatures which address the relationship between the AGT M235Tpolymorphisms and essential hypertension were searched in the PubMed(http://www.ncbi.nlm.nih.gov/pubmed/) and China Biological Medicine Database (CBM,http://cbm.imicams.ac.cn/) from the year1996to2011. For this meta-analysis to explore theassociation between the AGT M235T polymorphisms and essential hypertension was carried outusing Stata version11software.2. This study was case-control study involving1024essential hypertension patients and1024age-and gender-matched controls, aging35-70. SNPstream and RFLP-PCR were used to examinethe AGT tagSNP polymorphisms, and epidemiological and biochemical index were alsoinvesitgated. This study aimed to explore the association of the AGT gene polymorphisms andessential hypertension in Chinese Han population of Ningbo City.Results：1. The meta-analysis included results from38published literatures and one unpublished result.For AGT M235T polymorphism, the summary OR for TT/MM was1.69(95%CI:1.29-2.21) by random effects model. The summary OR for MT/MM genotype was1.06(95%CI:0.85-1.32) byrandom effects model. The possibility of publication bias is low.2. As compared with controls, smoking, drinking, height, weight, body mass index,triglyceride, total cholesterol were significantly associated with the occurrence of essentialhypertension (p <0.05), but high-density lipoprotein (HDL) was not. There were no significantassociation in the14tagSNP polymorphisms (p>0.05), which include rs699, rs11122575,rs2478543, rs1078499, rs3789671, rs7539020, rs7079, rs3889728, rs11122577, rs2478523,rs11568046, rs2493137, rs4628514and rs7536290. rs2493132, rs3789678, this twopolymorphisms were statistically significant (p <0.05).The study showed there was a statisticallysignificant gene-environmental interaction between rs3789678, rs2478523, rs7536290and BMI.Conclusion：1. The meta-analysis suggested that TT genotype of AGT M235T polymorphism might berelated to the risk of essential hypertension in Chinese Han population.2. Smoking, drinking, body mass index, triglycerides, and total cholesterol were the riskfactors of essential hypertension. There was a significant association between rs2493132,rs3789678polymorphisms and essential hypertension. In addition, there was a significantinteraction between rs3789678, rs2478523, rs7536290and BMI.