Effects Of Propranolol On Proliferation Of Hemangioma Endothelial Cells From Infantile

Objective To investigate the Effects of propranolol on proliferation ofhemangioma endothelial cells from infantile. Methods In this study,wetreated the vascular endothelial cells of hemangioma in96-well plates withdifferent dose(0μg/ml to120μg/ml)of propranolol.the survival and proliferatoinof the vascular endothelial cells of hemangioma were evaluated by the3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetra-zoloum(MTT) assay and crystalviolet assay. The effects of propranolol on the vascular endothelial cells ofhemangioma were observed by cell growth curves and flow cytometry. ResultsWith the augmentation of the concentration of propranolol (0μg/ml,20μg/ml,40μg/ml,60μg/ml,80μg/ml,100μg/ml,120μg/ml)and the lengthening ofaction time（24h,48h,72h）,proliferation of hemangioma endothelial cells areinhibiting apparently.AnnexinV-PI assay were aslo performed.propranolol couldinhibit the growth of vascular endothelial cells of hemangioma and advancingapoptosis. Conclusion Propranolol is able to inhibit the proliferation of thehemangioma endothelial cells, and to advance apoptosis of vascular endothelialcells,resulting in inhibition of hemangioma.