The Research Of The Treatment On Coccidiosis And The Safety Of The Amprolium Hydrochloirde、Ethopabate And Sulfaquinoxaline Soluble Powder

Coccidiosis is an important parasitic disease, which causes serious damage to thepoultry industry and it relies on anti-coccidiosis agent in prevention and control ofcoccidiosis.The two experiments were conducted in order to evaluate the effect of thetreatment on coccidiosis and the safety on the chicken of the AmproliumHydrochloride Ethopabate and Sulfaquinoxaline Soluble Powder.1Treatment test on coccidiosis of the Amprolium Hydrochloride、 Ethopabate andSulfaquinoxaline Soluble PowderFive hundreds healthy chickens of8years old were grouped into6tranches atrandom except the middle250only,while other groups were50in each，whichincluded the blank control group, positive control group, the high dose group(every1L water0.5g), the middle dose group(every1L water0.25g), the low dose group(every1L water0.125g)of the Amprolium Hydrochloride Ethopabate andSulfaquinoxaline Soluble Powder, and Dikezhuli (every1L water1g) controlgroup.All the chicken were vaccinated1.0×105E· tenella sporulated oocystsuspensions except the blank group.Meanwhile,the infection experiment groups weregiven Amprolium Hydrochloride Ethopabate and Sulfaquinoxaline Soluble Powdersolution and Qiuning by drinking water for a consecutive5days and the other twogroups were given water only.Taking the observation of spirit, appetite, stool changesafter the infection and count the bloody stool. The stool were collected and theoocysts were counted. in the seventh day.In the end of the experment,all the chickenswere killed and calculation anticoccidial index (ACI) to determine the efficacyaccroding to the standard of the United States Merck Co (1976) method and theMinistry of agriculture experimental clinical test specification (for TrialImplementation). The results shows that the low,the middle and the high dose allhave the effection on the coccidiosis.The best one is the middle dose,which wasmixed with1L water and0.25g Amprolium Hydrochloride Ethopabate and Sulfaquinoxaline Soluble Powder for a consecutive5days.2.Satey test of the Amprolium Hydrochloride、Ethopabate and SulfaquinoxalineSoluble Powder on the target chickensTwo hundreds and fifty healthy chickens which are8years old were grouped into6tranches of50in each group at random，which included solution recommended dosegroup(0.25g／L)，triple recommended dose group(0.75g／L)，5times recommendeddose(1.25g／L)，10times recommended dose(2.5g／L)，and control group．Theexperiment groups were given Amprolium Hydrochloride Ethopabate andSulfaquinoxaline Soluble Powder solution by drinking water for a consecutive21dayswhile the control group drinks water.All the chickens were weighted and calculatedthe feed utilization rate by the end of the test.And at the same time，hematology andblood biochemical indicators,pathological histology were examined and the generalclinical symptoms was observed.The results indicated that there are no significantchanges in hematology parameters between the experiment groups and controlgroup(p>0.05).There was no seeing any changes in all the indicators in below3timesrecommended dosage．However,ALP AST T-BIL D-BIL I-BIL of the5times and10times recommended dosage had the significant difference with the control(p<0.05)).However，we had observed the yellow liver and bleeding and bleeding spotsin intestinal in a number of chickens of the5times and10times recommended dosagegroup．The experments suggest that more than5times recommended dosage of theAmprolium Hydrochloride Ethopabate and Sulfaquinoxaline Soluble Powder is toxicfor the chickens’ healthy,mainly caused by yellow liver and bleeding spots inintestinal.5times recommended dose group and all had the degeneration and necrosisin liver and red cell exudation of intestinal mucosa, red cell exudated the intestinalmucosa through the histological observation.The individual chicken if10timesrecommended dose group of were also visible liver fatty degeneration.The testsindicated that the Amprolium Hydrochloride Ethopabate and SulfaquinoxalineSoluble Powder below the3times is safe to the target animal chicken.