The Study Of Genetic Diversity Of The Spring Soybean With SSR And SRAP Markers

In this article, we use77Germplasm resources most of which are from Sichuan, the genetic diversity and genetic relationship of the spring soybean were studied by SSR and SRAP markers. The results as following:1.30SSR primers and19SRAP primers were obtained respectively. Polymorphic percentage is100%and72.5%.127allelic variation were amplified by30reliable primers in SSR and the average was4.23, The Simpson diversity index (D) were0.2962to0.7634of SSR and the average was4.23, The Shannon diversity index was0.4226to1.4985of SSR and the average in each was0.9942.148allelic variation were amplified by19reliable primers in SRAP, the average in each was7.79. The Simpson diversity index (D) were0.6094to0.9098of SRAP, the average was0.8278. The Shannon diversity index was1.2357to2.0322of SRAP, the average was1.6955. These revealed that the selected materials have abundant genetic diversity.2. Calculated the genetic similarity coefficient with the datas of SSR, SRAP and their aggregation. The result of clustering base on UPGMA indicated that the similarity coefficient was between0.5942and0.9058. We used0.72as a cut-off point and the data could be classified into four groups.Group Ⅰ accounted for1%and contained1variety.It’s a developed cultivar come from Ya’an, because of the far relationship with cultivars we make it a single class;Group Ⅱ accounted for1%and contained1variety.It’s a developed cultivar come from Zi’gong, because of the far relationship with other cultivars we make it a single class;Group Ⅲ accounted for4%and contained3varieties,2of them was developed cultivars,1of them was germplasm materials.It’s a developed cultivar come from Liao’ning and Ya’an.Group Ⅳ accounted for94%and contained72varieties,59of them was developed cultivars,13of them was germplasm materials. It’s a developed cultivar come from Ya’an, Nanchong, Chengdu, Guning, Leshan and so on;The Group Ⅳ can be divided into six subgroups.First kind contained1variety Tiefeng25, which is come from Liaoning;second kind contained4varieties, they are fu2003-2and fu2008from Maoxian, gong89-1from Zigong and94-1from Ya’an; third kind also contained1variety xidou-3, which is come from Chongqing; fourth kind contained3varieties, they arechuandou-6和4515from Ya’an, banyedou-2from Chongqing;fifth kind contained3varieties,they are gong88-6from Zigong, nan107-23 from Nanchong and fu2003-2(breeding)from Maoxian; sixth kind contained60varieties.3. The correlation between the two sets of similarity parameters calculated with SSR and SRAP data respectively (r=0.5896, p<0.05), indicating that the two methods were differen. Maybe is the different of principle and the different function areas. While the similarity coefficient between SSR and SSR+SRAP is r=0.8429, p<0.01and between SRAP and SSR+SRAP is r=0.9316, p<0.01, both are matching well.Indicating that the SSR+SRAP can identification and classification the77spring soybean varieties better.