The Character Identification And Genetic Diversity Analysis With SRAP Maker In Sorghum/Sweet Sorghum Germplasm Resources

Sorghum (Sorghum bicolor L. Moench) belong to gramineae, was a annual herb naturalized in the Africa. Sorghum as a kind of efficient crop, has the ability of resisting to drought and waterlogging. At present, there are many different kinds of sorghum varieties. The study of the sorghum grain, stalk and other agronomic traits can promote to the effective use of sorghum/sweet sorghum germplasm resources, and provide a beneficial reference.to the full development and utilization of sorghum breeding.This research refer to 153 copies of sorghum and sweet sorghum varieties, including 78 copies of sorghum and 75 copies of sweet sorghumis which mainly imported from the United States, Africa and Chinese. The main content of this research was anlyze the data of sorghum economic characters and germplasm genetic diversity basis on clustering analysis and SRAP molecular cluster analysis, respectively. This research also has a preliminary study to sorghum origin and evolution. The purpose of this research was to providing scientific reference on choosing good varieties which have high sugar content and suitable for great culturing, new energy development. The main results were as follows: 1. The clustering analysis of agronomic traits shows that both sweet sorghum 1-1 and America 13-3 are with the highest yield of stalk and grain. Their spike shape are loose panicle, and are suitable for planting in the south. Africa stweet sorghum 2-1, America sorghum 17-1, Africa stweet sorghum 3-2, Africa stweet sorghum 1-2, America sorghum 1-4, America sorghum 13-2, and Shandong Weifang, all of them have relative good traits, which can be used as hybrid parents for breeding new variety.2. The result of the cluster analysis based on SRAP marking technology show that:with the average genetic distance of 0.675, we can divide the sorghum into six groups,including group A with 86 sorghum germplasm, group B with 52 sorghum germplasm, group C with 3 sorghum germplasm, groups D with 2 sorghum germplasm, group E with 6 sorghum germplasm,group F with 4 sorghum germplasm. This result show that the germplasm geography sources are different in the same group, and the same geography sources of germplasm are clustered in different groups.3.Sorghum germplasm resources SRAP marker genetic similarity analysis show that:the average genetic distance difference between 12T494 (Heilongjiang) and 12T421 (Heilongjiang), MN-3020(America) and MN-3378 (America), Africa sweet sorghum 3 (Africa) and Africa sweet sorghum 1-2 (Africa) are extremely small, indicating that the genetic relationship is very close.4. Sorghum germplasm resources SRAP marker genetic differentiation analysis show that:the genetic dissimilarity coefficient between Shandong Weifang(Shandong) and American sorghum 1-7 (USA), 97-8-F3-DF1SOB(Africa), Shandong Weifang(Shandong), ACHILLO (USA), are 0.8327,0.8526 and 0.8421, respectively. The genetic dissimilarity coefficient between Fujianl-1(Fujian) and MN-3382(USA),Jin mixed 23-5(Shanxi),Henan sorghum 2(Henan), aorg-Jude (Africa) are 0.8237?0.8317?0.8 and 0.8631, respectively.The more distant in genetic relationship between the above germplasm, the greater difference in genetic diversity they have. Thus, with different geographic origin, they can be used as parent material for genetic breeding.