| The goose is the waterfowl, mainly feeding green roughage-based, able to digest dietary fiber, and to obtain needed nutrients from the high-fiber feed with roughage resistance, strong resistance and other characteristics. Goose have ability about digest dietary fiber,it may provide a method to save the feed use of grain. The effects of dietary fiber digestion and utilization of capacity determined by its structure and function of the digestive system. Developed muscular stomach of geese can be disintegration and destruction of the plant cell wall, and make for digest the cell contents. But dietary fiber can not be enzymatic hydrolysis in higher animals, can only be degraded by microbial fermentation, generating volatile fatty acids to provide energy for the animal. In fact, no matter what kind of animals they use the most basic mechanism of dietary fiber is the use of microbial enzyme decomposition products or microbial metabolites. Thus, function of strain and cellulose decomposition in the Goose Road play a key role in the digestive use of dietary fiber. By constructing the Goose Road cellulose decomposing strains of the key features, provide a theoretical and practical basis for use of the effect of geese on the digestion of dietary fiber, the mechanism about digestion of the fiber substance of Goose, and improve the utilization efficiency of dietary fiberRight now, the reportes about effect of the digestion of dietary fiber are common more. But the mechanisms of dietary fiber digestion studies reported of goose still less. Domestic research focuses on the level of dietary fiber, dietary fiber sources. Some scholars try to add exogenous probiotic and enzyme preparation in the diet of geese, and received good results. It is not reports about this research in Foreign. The flora of the Goose Road is a very very complicated system, the function of the microbial flora of Goose Road, usually changed with his changes in its composition. With the rapid development of molecular biology techniques, we can study the dynamic changes of the Goose Road with denaturing gradient gel electrophoresis fingerprinting techniques,in this way, it provide a possible to confirm the member of the critical strain.after it, the key functions of strain can be separated, and the cellulose decomposition members of the critical strain can be constructed. In this study, we can study the impact of a high level of dietary fiber on microbial diversity of Goose Road with the technology of molecular biology PCR-DGGE, through the advantages of intestinal microbial flora of the normal dietary fiber group and a high level of dietary fiber groupchanges to reflect the relationship between microbial cellulose decomposition, and then filter out the composite flora of decomposition is closely related to cellulose and cellulose decomposition. Bioaugmentation to look forward to eventually achieve the rapid start-up and stable operation of the cellulose decomposition. Lay the theoretical and practical basis for the realization of the efficient use of cellulose.The test used bacterial16S rDNA of the V6-V8universal primers to amplify the total microbial DNA of rearing Jilin white goose normal diet group and a high level of dietary fiber group Goose Road, and then by denaturing gradient gel electrophoresis in accordance with each bowel comparison analysis. Three significant differences bands in the experimental group Myosoton Road on the DGGE gel were cut plastic, recycling, secondary DGGE, cloning and sequencing.using the BLAST software in GenBank sequence to compare the homology, drawn on the1st differential bands was highly similar to Escherichia coli16S rDNA sequence which have reported, the homology of up to99%, to confirm the No.1difference is Escherichia coli; he homology of the2nd differential bands and Enterococcus faecalis16S rDNA sequence reported have up to100%, to confirm the No.2differential bands was Enterococcus faecalis; he homology of the3rd of difference and the horse stomach aureus16S rDNA sequence reported have up to100%, to confirm the3rd differential bands eas the horse stomach aureus.Then, the separation of the Goose Road contents, Escherichia coli and Enterococcus faecalis go by Gram staining, screening, biochem ical tests, secondary identification,16S rDNA of the V6-V8region sequences of the PCR amplification and sequencing. And sequencing of the candidate strain results with the purpose of differences with the sequence alignment, eventually filter out the purpose of strain which formance of PCR-DGGE obvious travel the opposite sex.scherichia coli and Enterococcus faecalis, an important material for future study foundation. |
