Anaylsis Of Pestieide Multiresidues In Chinese Medicine Health Wine

Chinese medicine (CM) health wines are consumed as liquor in many areas of Asia. They play an important role in Asian life, culture and diet since ancient times. CM health wines, have not only high nutritive value and special flavor, but also health function. As is well-known, the raw materials of CM health wine are various Chinese material medicas (CMMs). The major components of CM health wines are alkaloids, salts, glycosides, organic acids and volatile oils. Because of increasing requirements of CMMs, organophosphorus pesticides (OPPs) are applied to these raw herbs to reduce disease and insect pests in their growth and production progresses. But, the overuse of pesticides in these progresses would lead to serious pollution on CMMs, further to contaminate CM health wines.Both the quality and safety of wine have been of growing interest for consumers all over the world, with an increasing emphasis on health risks from food in the public debate. Pesticide residues are not directly addressed into wine, but it is generally regulated through the different national and regional standards for natural medicines as maximum residue limit (MRLs). The European Union (EU) has set MRLs for pesticide residues in wine grapes (0.01-10mg kg-1depending on the particular pesticide).OPPs are the most frequently used pesticides worldwide. Some of them may persist in the environment to contaminate soil, air, surface and ground water. The incorrect use or overuse of OPPs may result in the residues of these compounds in agricultural products and derivative food commodities, such as wine, fruit juices. So, it is necessary to detect and control the OPP residues in raw CCMs and their products including CM health wine for food and environmental safety, which plays an important role in food quality for evaluating food safety and possible risks to human health.Various analytical methods including gas chromatography (GC) and liquid chromatography (LC) coupled with various detectors have been developed for the determination of pesticides in wine samples. Among these methods, gas chromatography with flame photometric detection (GC-FPD) is the most frequently used techniques. Before the analysis of pesticides by GC-FPD, an important and critical step is the sample extraction and clean-up. Different extraction and clean-up procedures, such as solid phase extraction (SPE), liquid-liquid extraction (LLE) solid-phase micro-extraction (SPME), have been established for extracting numerous pesticides in wines. However, SPE and LLE require large amounts of organic solvent in pretreatment, which may lead to large waste of solvent and serious contamination to the environment. SPME often results in a non linear response due to complex matrices. Also, the clean-up step may result in the loss and damage of target analytes. Therefore, a simple one-step extraction procedure using a little solvent without any further cleanup steps is a good selection.By the developed GC-FPD analytical method,18OPPs in Chinese medicine health wine samples were successfully detected. As a screening method, this methodology has the advantage of being simply, easy and rapid with high extraction recoveries, also the values of LOD were below the MRLs of wine grapes imposed by England and European legislation. Total analysis time is50min (10min for extraction plus39min for chromatography). Thus,6samples can be prepared in less than1h. Due to the optimized extraction procedure and the chromatographic conditions as well as omitting any cleanup step, the method could be expanded to even further analytes depending on the availability of standards and could be used in routine analysis. It should also be noticed that positive cases should be confirmed by a complementary detection method, or the analysis performed by GC-MS.In the present study, a rapid, reliable and simple extraction for GC-ECD determination of27Organic Chlorine Pesticides (OCPs) and pyrethroid insecticides in80CM health wines was successfully established. To the best of our knowledge, this is the first report of multi-residue determination of OPPs in CM health wines, providing references for monitoring the quality of CM health wine in routine analysis.This article studied the analytical method of pesticide residue in CM health wines. And this article could to make a greater contribution to the pesticide residues of the standard in CM health wines.