Polymorphisms Of Inner Mongolia Cashmere Goats Keratin14(K14) Gene Promoter And Relevance With Their Wool Trait

Keratin14(keratin14, K14) is a one of the family of keratin expression in epidermal basal cells and hair follicle outer root sheath cells and Ⅱ keratin K5pairs children are considered biomarkers of the overlying stratified squamous epithelium matter. The study found that the gene promoter regulates the transcription start and rate, thus affecting the expression of the gene, so we carried out relevant research (?) the Inner Mongolia Cashmere goats keratin14promoter.Test one:Cloning and analysis of goat keratin14promoter sequence. In th(?) study, the first of Inner Mongolia Cashmere goats DNA as a template, the reference t the cattle K14sequence design and synthesis of primers. PCR amplification to obtai the length of2834bp (Registry Number:JQ063036), including start-2631bp and th(?) structural gene of the promoter region of the first exon District203bp goat K.14gen(?) fragments, the use of bioinformatics methods to analyze the structure of the Inner Mongolia Cashmere goats keratin14promoter, and made a comparison with human and bovine K14promoter sequences. Results:the goat K14promoter structure and human K14promoter different, containing only Sox,-5, GATA-1, GAT A-1/2binding sites of three common transcription factor binding sites. And cattle have a higher similarity, containing Sox,5,7of the SRY, Cdx A, Myo D, MZF1, GATA-1/3, Nkx-2binding points common transcription factor binding sites. And goat K14promoter region delta E, GATA-2and GATA-1, Sp-1AML-la,5unique transcription factor binding sites.Test two:The goat K14gene single nucleotide polymorphism analysis.(1)By20goats mixed DNA as a template to find the polymorphic loci on the basis of the Inner Mongolia Cashmere goats keratin14promoter molecular cloning, sequencing results showed that the primers D1and D3amplified fragment-421and-1838at the existence of sets of peaks.(2)By PCR-SSCP and DNA sequencing techniques detect molecular variation of the Inner Mongolia Cashmere goats keratin14promoter at-421and-1838were designed primers S1and S2, as well as Inner Mongolia Cashmere cashmere wool shape doanalysis.150Inner Mongolia White Cashmere goats for the study, using PCR-SSCP polymorphism in the K14promoter. Results:in the primers S1amplification product of AA, AG, the GG3genotypes; primers S2amplification product of the CC, CT, and TT genotypes; Cloning and Analysis of sequencing analysis on these two fragments, respectively. of the-421G→A,-1838C→T base sequence mutation.(3) population genetics show that:test the-421G→A and-1592C→T polymorphic loci by χ2the SNPs at Hardy-Weinberg,(P>0.05) equilibrium;-421(G→A) sites of the AA genotype velvet thickness was significantly lower than for GG and AG genotype, the fiber length of the GG genotype was significantly higher than the AA genotype. Hair long and capillary of the TT genotype of the-1838(C→T)should be significantly higher than the CT genotype and CC genotype, and long hair of which the TT genotype CC genotype is more relative to the CT, capillary relative to the CC, CT genotype more pronounced.