The Effect Of Glutamate On Weaned Piglet Nutrition And Intestinal Nervous System

In order to explore the effect of Glu on enteric nervous system and the nutrition of weaning piglets, weaned piglets on the28d age were fed with the basal diet and diet added1%Glu in this study. Growth performance, biochemical indicators, serum amino acids, observation of intestinal slices and the expression of related genes were tested. Results shows that supplement of1%Glu has no obvious effect on average daily feed intake and average daily gain were not significant (p>0.05). Supplement of1%Glu in diet can decrease feed conversion ratio, pancreas index and lean meat percentage declined (p>0.05), while enhancing renal index (p<0.05), heart, liver, spleen index (p>0.05) and fat rate increased (p<0.05). When treated with1%Glu, serum GH was found significantly increased, while TNF and EGF decreased (P<0.05). The content of HDL, U, ALB, GLU, LPS and TP in serum Increased, while the content of IGM, LDH, ALT and AST, CK, the LAC decreased (P<0.05). Higher concertration of serum Pro, Ala, Arg and lower Asp (P<0.05), Glu (P>0.05) were found in added1%Glu group than that in the control group after10days supplement of1%Glu. Continue feeding to20days, in1%Glu group, serum Thr (P<0.05), Ala (P<0.05) and Gly (P>0.05) was increased, while other amino acids in serum were no significant differences. Supplement of1%Glu can enhance the jejunum villus height (P<0.05), villus width (P<0.01) significantly and increased crypt depth (P>0.05), but has no effect on the ileum, the three indicators (P>0.05). Supplement of1%Glu can also promote the expression of EACC1Glt1and C-fos significantly in the jejunum (P<0.05), the expression of C-fos in the kidney (P<0.05), the expression of EACC1in the ileum while decrease the expression of Glt1in the kidney (P<0.05).In conclusion, supplement of1%of Glu in diets can promote the healthy growth of the weaned piglets, reduce the stress response, improved feed conversion rate, balance the body amino acids and at the same time can protect neurons in the enteric nervous system. The results of this study will help to evaluate Glu utilization, explore the regulation of intestinal Glu metabolism, improve the efficiency of the feed Glu nutrition, and provide a new thinking way for the study of the role of Glu on intestinal nervous system.