The Effect Of Replacing Antibiotics Feed Additive With Gluconic Acid, Glutamine And Their Combination On Production Performance And Immune Of The Piglets

The objective of this article was to study the effect of replacing antibiotics feed additive with gluconic acid, glutamine and their combination on production performance and immune and so on of the piglets in intensive production conditions.24litters of1-day-old piglets were selected and randomly divided into four groups with6replicates per group, experiment lasted for42days. The control group was fed basal diet added antibiotic (60mg/kg colistin,75mg/kg chlortetracycline); the test group was fed the basal diet added0.15%GA,0.2%Gln,0.15%GA+0.2%Gln, individually. Piglets were weaned at28d of age, the litter was used as replicate.14days after weaning, one pig was randomly chosen from each litter, Collect blood from the front vena cava, for preparing serum. And then slaughter, open the abdominal cavity, separating the stomach, liver, kidney, spleen, weighing and determine the pH of the stomach contents; separate intestine and collect the contents, and determination the pH value, fix part of the intestine, the remaining intestine part were cryopreserved.The result showed that the replaced groups had no significant difference with the antibiotics feed group on the average daily feed intake, the average body weight, the feed conversion ratio and the death and culling ratio, but the average daily gain was worse than the antibiotics group from21days to42days; at the same time, compared with the piglets in the antibiotics feed group, a gradually decreasing trend on diarrhea head times in the replaced group.The GA group and GA+Gln group increased the index of duodenum, ileum, liver(P>0.05), Gln group increased the index of kidney, the ileal index in Gln group was significantly lower than that in the GA+Gln group(P<0.05). Compared with the antibiotics group, the replaced group decreased the crypt depth of the duodenum, and increased the mucosal protein content in the duodenum and jejunum, but the content of alkaline phosphates in the intestinal mucosa decreased.Compared with the antibiotics group, the trial group increased the the number of bacteria in the cacum and colon, decreased the pH of the cacum and colon content; the trial group increased the content of lactic acid in the ileum, cacum and colon.Compared with the control group, the GA(P<0.05), Gln(P<0.05), GA+Gln(P<0.01) group improved the content of lysozyme in the jejunum, GA+Gln group increased the lysozyme in the duodenum, ileum, but had no significant difference(P>0.05). And the trial group increased IgG content in serum(P>0.05).Compared with the control group, the alkaline phosphates activity was decreased in trial group, increased the total protein and globulin numbers in serum. There was no significant difference on the albumin, sugar, trilycerides content(P>0.05). In our trial, GA decreased the urea content.Collectively, the piglets in the antibiotics-treated group compared with the control group, there is a decreasing trend about the diarrhea frequency, increase the total protein and globulin content in serum, increased lysozyme content in jejunum, bacteria number in cacum and colon, and lacric acid production have a increased trend, decread crypt depth in duodenum and back jejunum, and the pH in cacum and colon content.