Effects Of Phytase And Compound Enzymes On Growth Performance And Nutrients Utilization In Kunming Mice

Two experiments were conducted to determine the effects of phytase and compound enzymes on growth performance、nutrients apparent digestibility and serum biochemical indicator in Kunming mice.Experiment1:90SPF male mice with similar weight were chosen and assigned randomly to five treatment groups, including a control group and four experimental groups, each group with three replicates. The control group calcium phosphate dibasic level was0.80%without phytase, four experimental groups calcium phosphate dibasic levels were0.60%,0.40%,0.20%and0.00%with500U/kg phytase addition, respectively. The results were as follows:(1) The ADG, ADFI and A/G were not significantly different between control group and experimental groups(P>0.05).(2) Compared with control group, the apparent digestibility of DM, CP and EE of experimental groups were increased except no calcium phosphate dibasic, but they were not significantly different among treatment groups(p>0.05), the apparent digestibility of Ash were improved(p>0.05), the apparent digestibility of Ca of0.60%and0.00%calcium phosphate dibasic groups increased32.00%(p<0.05) and44.56%(p<0.01), respectively. The other experimental groups did not differ significantly with control group(p>0.05). The apparent digestibility of TP of0.40%,0.20%and0.00%calcium phosphate dibasic groups were increased significantly(p<0.01),0.60%calcium phosphate dibasic group was also improved(p>0.05). The treatment groups had the decreasing tendency to the TP of droppings in response to descreasing calcium phosphate dibasic levels in diets, and the the TP of0.40%,0.20%and0.00%calcium phosphate dibasic groups were decreased significantly(p<0.01).(3) The serum biochemical indicator of treatment groups did not differ significantly(p>0.05).Experiment2:108SPF male mice with similar weight were chosen and assigned randomly to six treatment groups, including a control group and five experimental groups, each group with three replicates. The control group CP level was21.41%without compound enzymes, five experimental groups CP levels were20.88%,20.30%,19.94%,19.32%and19.00%with0.10%compound enzymes addition, respectively. The results were as follows:(1) Compared with control group, The ADG and ADFI of experimental groups were increased at0to2weeks(p>0.05), and decreased at3to4weeks(p>0.05). The A/G of treatment groups did not differ significantly(p>0.05).(2) Compared with control group, the apparent digestibility of DM, CP, Ash, Ca and TP of experimental groups were increased, and20.88%CP group increased significantly at the apparent digestibility of DM, Ash, Ca and TP(p<0.05). The CP of treatment groups did not differ significantly in droppings(p>0.05).(3) The serum biochemical indicator of treatment groups did not differ significantly(p>0.05).In conclusion:(1) In experiment1conditions,500U/kg phytase can replace100%calcium phosphate dibasic in the diet of Kunming mice under the condition of growth performance, nutrients apparent digestibility and serum biochemical indicator, and reduced the P levels in mice droppings(p<0.01).(2) In experiment2conditions, added0.10%compound enzymes can replace2.41%CP in the diet of Kunming mice under the condition of growth performance, nutrients apparent digestibility and serum biochemical indicator, but can’t reduced the CP levels in mice droppings(p>0.05).