Effects Of β-galactomannan On Enzyme Activity Related Energy Metabolism And Immune Function Of IPEC-J2 Or Kunming Mice

The purpose of this test was to investigate the effect ofβ-galactomannan on energy metabolism and immune response in the body.Firstly,IPEC-J2 cells were treated with different concentrationsβ-galactomannans to determine the reducing sugar content and reducing sugar change rate in the culture medium,cell viability,enzyme activity related to energy metabolism,and expression of cytokines.In order to further verify the effect ofβ-galactomannan on the body’s energy metabolism and immune response,24 healthy 5-week-old male Kunming mice were selected and randomly divided into 4 treatment groups,namely the control group,1000μg/m L,2000μg/m L and 4000μg/m L group,respectively according to the mouse body weight of 10 m L/kg·d^-1 sterile water,1000,2000 and 4000μg/m Lβ-galactomannan solutions,3 replicates with 2 mice each.Feed and drink freely during the whole process,adapt to 7 days,and formally test for 7 days.During the experiment,the body weight and feed intake of the mice were recorded,and the average daily weight gain and average daily feed intake were calculated.After the test,the organs of the mice were weighed to calculate the organ index;blood and liver were collected,and the content ofα1-acid glycoprotein in serum and liver homogenate was measured;each of the duodenum,jejunum and ileum were taken to measure intestinal energy metabolism-related enzyme activity and cytokine expression.The test results are shown below:1.Treatment of IPEC-J2 cells with 1000,2000 and 4000μg/m Lβ-galactomannan for 0-2h accelerated the reduction of reducing sugar content and significantly increased the reducing sugar change rate in the culture medium（P<0.05）and significantly reduced the cell viability of IPEC-J2 cells at 1h and 2h（P<0.05）;2.1000μg/m Lβ-galactomannan significantly increased the activity of lactate dehydrogenase（LDH）and succinate dehydrogenase（SDH）（P<0.05）and significantly increased the expression of TNF-α,IL-6,IL-8 m RNA in IPEC-J2 cells（P<0.05）;3.Compared with the control group,theβ-galactomannan groups administered with gavage at 1000,2000 and 4000μg/m L significantly reduced the end-of-test weight of mice（P<0.05）and the average daily weight gain of the 1000 and 4000μg/m L groups significantly decreased（P<0.05）,in which the 2000μg/m L group had a significant downward trend（P=0.087）,and the feeding of different concentrations ofβ-galactomannan solutions had no significant effect on the average daily feed intake（P>0.05）;4.Compared with the control group,theβ-galactomannan group administered with gavage at 1000,2000 and 4000μg/m L significantly increased the spleen index and epididymal fat of mice（P<0.05）.The 1000 and 2000μg/m L groups significantly reduced the liver index,and subcutaneous fat content（P<0.05）;5.Compared with the control group,theβ-galactomannan groups administered with gavage at 1000,2000 and 4000μg/m L significantly increased the content ofα1-acid glycoprotein（α1-AGP）in the serum of mice（P<0.05）.2000and 4000μg/m Lβ-galactomannan significantly increasedα1-acid glycoprotein content in mouse liver（P<0.05）;6.Compared with the control group,the lactate dehydrogenase（LDH）enzyme activity in the mouse duodenum,jejunum and ileum of theβ-galactomannan groups was not significantly affected（P>0.05）,which was significantly increased by 2000μg/m L group in duodenum,jejunum and ileum and 4000μg/m L group of jejunum（P<0.05）;1000μg/m Lβ-galactomannan mouse in duodenum,jejunum and ileal of succinate dehydrogenase（SDH）enzyme activity had no significant effect（P>0.05）,significantly increased duodenum and ileum in the 2000μg/m L group and duodenum and jejunum in the 4000μg/m L group（P<0.05）;7.Compared with the control group,the 1000μg/m Lβ-galactomannan group significantly increased the expression of duodenum TNF-αm RNA（P<0.05）,the 2000μg/m L group of duodenum had a significant downward trend in the expression of TNF-αm RNA（P=0.084）and significantly increased the expression of IL-1αm RNA（P<0.05）,the expression of IL-1αand IL-6 m RNA in the 4000μg/m L group was significantly increased（P<0.05）for duodenum;1000μg/m Lβ-galactomannan solution significantly increased jejunum IL-1αm RNA expression（P<0.05）,2000μg/m L group significantly increased IL-1αand IL-6 m RNA expression（P<0.05）in jejunum,the 4000μg/m L group significantly increased jejunum TNF-αm RNA expression（P<0.05）;1000 and 2000μg/m Lβ-galactomannan solution significantly increased ileum IL-1αm RNA expression（P<0.05）,The group of 4000μg/m L significantly increased the expression of TNF-α,IL-1αand IL-6 m RNA（P<0.05）in ileum.In summary,cell test in vitro results show thatβ-galactomannan reduces cell viability,induces intestinal immune response,and improves the utilization of reducing sugars in the culture medium and the level of cell energy metabolism.On the other hand,Kunming mice were used to tests in vivo,and the results showed thatβ-galactomannan induced an intestinal inflammatory response and promotes the body to a stress state,thereby increasing the animal’s intestinal energy metabolism level and reducing growth performance.At the same time,as the dose ofβ-galactomannan increases,the body’s inflammatory response worsens and energy metabolism increases.