Genetic Diversity Analysis Between Aspergillus Flatus Resistant And Susceptible Peanut Species Baced On SSR Markers

Peanut is one of the most vulnerable to infection of aflatoxin crops in our country,China is also one of the countries where the aspergillus toxin pollution is very serious.It is necessary to strengthen the aflatoxin genetic resources evaluation and todisscover excellent species resistant to aspergillus thus build advanced screeningtechnology. Most of the research with genetic diversity of peanuts focused onagronomic characters, and very few studies review the genome DNA level of geneticdiversity. With the selection of the40species resistant to aspergillus, which wereoperated by SSR molecular markers. Showing the genetic diversity of DNA level ofpeanuts, this paper reveals the genetic variation and relationships of germplasmresources. The results show that:(1) screened for SSR molecular markers of genomic DNA extraction methods,using the improved CTAB method to extract DNA genome can completely satisfy theexperiments. Through treatment of isopropanol and precooling70%ethanol solutioncan greatly improve the efficiency of the extraction and improve the quality of DNA.The extraction method suits mass and rapid extraction of plant genome DNA.(2) There are rich polymorphism s exist in peanut. This trial got35primers ofpolymorphism thus expansion of229bands. Including169polymorphisms bands,account for73.8%of the total bands. Alleles on each locus generally from2to12,average5.51.In which PM15with15bands gets the maximum number of bands, theleast is PM375, only three.(3) The PIC value of the35primer ranges from0.305-0.843. Only a few primersamong35points (PM188, pPGPseq-4H11, Ah3, RM14E11, gc25, gc53, GNB837,GNB613) is less than0.5. Explaining that the selected marker has highpolymorphisms can accurately reflect the breed relationship between genetic orspecies in molecular level.(4) These35primers can separate40peanuts material generally. Using the method of NTSYSpc2.1UPGMA to SHAN clustering analysis of SSR results. Plotclustering analysis Tree shows that all materials can be divided into two categorieswhen the threshold value is0.56. It proves the existence of peanuts resistant toaflatoxin in both materials. When the coefficient number is0.71, the population canbe divided into five groups: resistant varieties AR-1, AR-2, KB153, KB154, XW84,XW85, XW87, KB113, KB157, UF71533-1, XW83, XW101, XW88, AR-3, andICGV GFA-A series and Xin Hui Xiao Li were placed in A group because thecoefficient of similarity is very high; Jin Hua1012and Bai Sha1016was also placedin one group; Xiangxiang and AR-4were in the third group; ICGV92196was placedalone; Zhan Qiu48and Quan Hua10were in the fifth group. Clustering analysisshows that the geographical factors is the crucial factor for the formation of varieties.