Effect Of Achyranthes Bidentata Polysaccharides On Nutrition And Immune Function In Ctenopharyngodon Idellus

To investigate nutritional effects and immune function of Achyranthes bidentata polysaccharides (ABPS) on grass carps, five test groups were used, which consisted of control group,0.05%,0.10%,0.20%and0.40%ABPS addition group.375grass carps with average body weight (79.7+2.4) g were randomly allocated to five groups with three replicates and with25grass carps in each replicate and were fed for65d.Results are as bellow:1. The viscerosomatic index was increased significantly in0.20%and0.40%(p<0.05),and0.20%was the best level;but the composition of the liver was not significantly affected(p>0.05);the body moisture and crude protein had the highest content in0.20%level,but the difference was not significant(p>0.05);the content of crude fat was significantly increased in all levels(p<0.05),but0.40%had the most significant effect; the activities of intestinal protease,amylase and foregut lipase had the most significant differerce compared with the control group in the0.20%level(p<0.05),the activities of midgut lipase was the highest in0.20%level,too,but the difference was not significant(p>0.05).2.In0.10%level,the spleen somatic index was significantly higher than other groups(p<0.05);all the levels did not significantly affected the number of NBT positive cells(p>0.05),but the number of NBT positive cells in0.20%level was maximum; in0.20%level,T3,T4and Glu of serum were all increased,but did not achieve significant results (p>0.05);in0.40%level,TP and INS were reached to significant effect (p<0.05),and ALB was reached to maximum,but did not achieve a significant effect (p>0.05);in0.20%group,TNF-a,SOD.ACP and LSZ were reached to maximum and achieved significant difference (p<0.05),MDA was reached to minimum, and also showed significant difference (p <0.05), IL-1and CAT was maximum, but the differences were not significant (p>0.05);in0.40%level,ALP was reached to maximum and showed significant difference (p<0.05), IL-6was also reached to maximum.but the difference was not significant (p>0.05).3.Through a comprehensive analysis of the above conclusions,author draw that adding 0.20%Achyranthes bidentata polysaccharides to the basal diet of grass carp could significantly increase nutritional effects and immune function of grass carp.